Protective Effect of Hemin Against Experimental Chronic Fatigue Syndrome in Mice: Possible Role of Neurotransmitters

Autor: Mandeep Kumar, Puneet Kumar, Vikrant Rahi, Vandana Thakur, Sumit Jamwal
Rok vydání: 2020
Předmět:
0301 basic medicine
Dopamine
Protoporphyrins
Anxiety
Toxicology
medicine.disease_cause
Elevated Plus Maze Test
Lipid peroxidation
Mice
Norepinephrine
chemistry.chemical_compound
0302 clinical medicine
Enzyme Inhibitors
Neurotransmitter
Neurotransmitter Agents
Fatigue Syndrome
Chronic
Behavior
Animal
Hand Strength
Electron Transport Complex II
General Neuroscience
Brain
Hydroxyindoleacetic Acid
Glutathione
Mitochondria
Hemin
Locomotion
medicine.drug
Serotonin
medicine.medical_specialty
Metalloporphyrins
03 medical and health sciences
Internal medicine
medicine
Chronic fatigue syndrome
Animals
Nitrites
Electron Transport Complex I
business.industry
Homovanillic Acid
medicine.disease
Disease Models
Animal
030104 developmental biology
Endocrinology
chemistry
3
4-Dihydroxyphenylacetic Acid
Lipid Peroxidation
business
Heme Oxygenase-1
030217 neurology & neurosurgery
Oxidative stress
Behavioural despair test
Zdroj: Neurotoxicity Research. 38:359-369
ISSN: 1476-3524
1029-8428
Popis: Chronic fatigue syndrome (CFS) is a disorder characterized by persistent and relapsing fatigue along with long-lasting and debilitating fatigue, myalgia, cognitive impairment, and many other common symptoms. The present study was conducted to explore the protective effect of hemin on CFS in experimental mice. Male albino mice were subjected to stress-induced CFS in a forced swimming test apparatus for 21 days. After animals had been subjected to the forced swimming test, hemin (5 and 10 mg/kg; i.p.) and hemin (10 mg/kg) + tin(IV) protoporphyrin (SnPP), a hemeoxygenase-1 (HO-1) enzyme inhibitor, were administered daily for 21 days. Various behavioral tests (immobility period, locomotor activity, grip strength, and anxiety) and estimations of biochemical parameters (lipid peroxidation, nitrite, and GSH), mitochondrial complex dysfunctions (complexes I and II), and neurotransmitters (dopamine, serotonin, and norepinephrine and their metabolites) were subsequently assessed. Animals exposed to 10 min of forced swimming session for 21 days showed a fatigue-like behavior (as increase in immobility period, decreased grip strength, and anxiety) and biochemical alteration observed by increased oxidative stress, mitochondrial dysfunction, and neurotransmitter level alteration. Treatment with hemin (5 and 10 mg/kg) for 21 days significantly improved the decreased immobility period, increased locomotor activity, and improved anxiety-like behavior, oxidative defense, mitochondrial complex dysfunction, and neurotransmitter level in the brain. Further, these observations were reversed by SnPP, suggesting that the antifatigue effect of hemin is HO-1 dependent. The present study highlights the protective role of hemin against experimental CFS-induced behavioral, biochemical, and neurotransmitter alterations.
Databáze: OpenAIRE
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