Protective effect of surface layer proteins isolated from four Lactobacillus strains on hydrogen-peroxide-induced HT-29 cells oxidative stress
| Autor: | Bin-Bin Zhao, Ting-Ting Kang, Jun Meng, Rong-Rong Lu, Qiu-Xiang Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Intracellular Space Apoptosis medicine.disease_cause Biochemistry Antioxidants Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Bacterial Proteins Structural Biology Lactate dehydrogenase medicine Humans Molecular Biology Membrane Potential Mitochondrial biology General Medicine Hydrogen Peroxide Malondialdehyde Molecular biology Lactobacillus Oxidative Stress 030104 developmental biology chemistry Catalase 030220 oncology & carcinogenesis biology.protein Calcium Reactive Oxygen Species HT29 Cells Intracellular Oxidative stress |
| Zdroj: | International journal of biological macromolecules. 102 |
| ISSN: | 1879-0003 |
| Popis: | The objective of this study was to explore the antioxidant effect of the surface layer proteins (SLPs) and their mechanism. We investigated four SLPs which were extracted from L. casei zhang, L. rhamnosus, L. gasseri and L. acidophilus NCFM respectively using LiCl. The protective effect of SLPs on H2O2-induced HT-29 cells oxidative injury was investigated. As results, SLPs (100μg/mL) could significantly mitigate HT-29 cells cytotoxicity, improve the activities of total antioxidant capacity (T-AOC), catalase (CAT) and superoxide dismutase (SOD), decrease the contents of malondialdehyde (MDA) and lactate dehydrogenase (LDH), compared with H2O2-induced group (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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