Entry into S phase is inhibited in human fibroblasts by rat liver poly(A) + RNA
| Autor: | J.Keith McClung, C.K. Lumpkin, James R. Smith |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
DNA Replication
Microinjections Kinetics Biology Centrifugation Density Gradient medicine Animals Humans RNA Messenger Fibroblast Interphase Microinjection Mitosis Messenger RNA DNA synthesis RNA Cell Biology Fibroblasts Molecular biology Rats medicine.anatomical_structure Liver Biochemistry Cell culture Poly A |
| Zdroj: | Experimental Cell Research. 160:544-549 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(85)90201-0 |
| Popis: | Initiation of DNA synthesis was inhibited in human fibroblasts following microinjection with poly(A) + RNA derived from normal rat liver. After sucrose gradient sedimentation of the RNA, the inhibitory activity was found to be limited to two adjacent fractions. Dilution experiments suggest a minimum abundance level of 0.015% for this mRNA(s). Studies on the kinetics of this inhibition indicate a reversible inhibition with a duration of approx. 10 h. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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