Viability and gene expression responses to polymeric nanoparticles in human and rat cells
| Autor: | Alain Le Faou, Mosaad A. Abdel-Wahhab, Bertrand H. Rihn, Jérôme Chevrier, Olivier Joubert, Justine Paoli, Roudayna Diab, Carole Ronzani, Ramia Safar |
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| Přispěvatelé: | Cibles thérapeutiques, formulation et expertise pré-clinique du médicament (CITHEFOR), Université de Lorraine (UL), Faculté de Médecine [Nancy], National Research Center (NRC), National Research Center |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cell type
Cell Survival Health Toxicology and Mutagenesis [SDV]Life Sciences [q-bio] Cell Gene Expression Apoptosis 02 engineering and technology Biology Toxicology Cell Line 03 medical and health sciences chemistry.chemical_compound Polymethacrylic Acids medicine Autophagy Animals Humans 030304 developmental biology Cell Proliferation Inflammation 0303 health sciences Cell growth Cell Biology 021001 nanoscience & nanotechnology Molecular biology Rats Oxidative Stress medicine.anatomical_structure chemistry Cell culture Immunology Nanoparticles Trypan blue Tumor necrosis factor alpha 0210 nano-technology |
| Zdroj: | Cell Biology and Toxicology Cell Biology and Toxicology, Springer Verlag, 2014, 30 (3), pp.137-46. ⟨10.1007/s10565-014-9275-4⟩ |
| ISSN: | 0742-2091 1573-6822 |
| DOI: | 10.1007/s10565-014-9275-4⟩ |
| Popis: | International audience; Applications of polymeric nanoparticles (NP) in medical fields are rapidly expanding. However, the influence of polymeric NP on cell growth and functions is widely underestimated. Therefore, we have studied cell and polymeric NP interactions by addressing two cell types with two endpoints (viability and gene expressions). Rat NR8383 and human THP-1 monocytic cell lines were exposed to 6 to 200 μg/mL of Eudragit(R) RL NP for 24 h, and cellular viability was estimated using MTT, WST-1, and trypan blue tests. A decrease of viability was observed with NR8383 cells (down to 70% for 200 μg/mL), and on the contrary, an increase with THP-1 cells (up to 140% for 200 μg/mL). Differential expression of genes involved in oxidative damage (NCF1), inflammation (NFKB, TNFA, IL6, IL1B), autophagy (ATG16L), and apoptotic balance (PDCD4, BCL2, CASP8) was analyzed. ATG16L, BCL2, and TNFA were up-regulated in NR8383 cells, which are consistent with an induction of autophagy and inflammation. On the other hand, NCF1, NFKB, and IL1B were down-regulated in THP-1 cells, which may contribute to explain the increase of cellular viability. Our results show that (1) the toxic potency of NP is dependent on the cellular model used and (2) mechanistic toxicology should be the corner stone for the evaluation of NP hazard. |
| Databáze: | OpenAIRE |
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