Triiodothyronine regulates distribution of thyroid hormone receptors by activating AMP-activated protein kinase in 3T3-L1 adipocytes and induces uncoupling protein-1 expression
| Autor: | Dan Wei, Mei-Ping Guan, Cheng-Zhi Wang, Yao-Ming Xue |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Clinical Biochemistry AMP-Activated Protein Kinases Biology Ion Channels Mitochondrial Proteins Mice AMP-activated protein kinase 3T3-L1 Cells Internal medicine Brown adipose tissue Adipocytes medicine Animals Uncoupling protein RNA Messenger Molecular Biology Uncoupling Protein 1 Receptors Thyroid Hormone Triiodothyronine Thyroid hormone receptor Gene Expression Regulation Developmental AMPK Cell Differentiation Thermogenesis Cell Biology General Medicine Thermogenin medicine.anatomical_structure Endocrinology biology.protein |
| Zdroj: | Molecular and Cellular Biochemistry. 393:247-254 |
| ISSN: | 1573-4919 0300-8177 |
| Popis: | The purposes of this study were to examine whether thermogenesis in 3T3-L1 adipocytes is related to variations in thyroid hormone receptors (TRs) that are differently regulated by triiodothyronine (T3), and the possible role of AMP-activated protein (AMPK) in thermogenesis after cell differentiation. Differentiated 3T3-L1 adipocytes were maintained under four conditions: normal control group, T3 treatment group, AMPK agonist (5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside) treatment group, and T3 and AMPK inhibitor (Compound C) treatment group. Real-time polymerase chain reaction was then performed to evaluate the changes in TRα and TRβ mRNA levels in the cells, as well as marker genes for brown adipose tissue including uncoupling protein (UCP)-1 and Cidea. Western blotting was carried out for the cells to detect the expressions of TRα, TRβ, and AMPK protein levels. After T3 treatment, the mRNA and protein levels of TRα decreased compared with the control group, while TRβ mRNA and protein levels increased markedly at the same time. We also found elevated mRNA levels of UCP-1 and Cidea after exposure to T3. However, the distribution of TRs was reversed by Compound C. AMPK protein levels were clearly activated by T3. Our results suggest that the distribution of TRs is related to thermogenesis, and AMPK may participate in the alterations. |
| Databáze: | OpenAIRE |
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