Oesophageal squamous cell neoplasia in head and neck cancer patients: upregulation of COX-2 during carcinogenesis
| Autor: | H. Scherer, Hans Scherübl, Bodo Hoffmeister, B von Lampe, Bernhard Heine, Harald Stein, P Däubler, B Barthel, Kerstin Maaser |
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| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Esophageal Neoplasms Transcription Genetic Biology medicine.disease_cause Gene Expression Regulation Enzymologic medicine Humans RNA Messenger Esophagus Prospective cohort study Molecular And Cellular Pathology DNA Primers Base Sequence Head and neck cancer Membrane Proteins COX-2 medicine.disease Epithelium Gene Expression Regulation Neoplastic Isoenzymes medicine.anatomical_structure oesophageal squamous cell carcinoma Oncology Epidermoid carcinoma Cyclooxygenase 2 Head and Neck Neoplasms Prostaglandin-Endoperoxide Synthases Dysplasia Carcinoma Squamous Cell Immunohistochemistry head and neck cancer Carcinogenesis carcinogenesis |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Patients with (previous) head and neck cancer (HNC) are at high risk for developing second squamous cell cancer of the oesophagus. The role of cyclooxygenase-2 (COX-2) in oesophageal squamous carcinogenesis has not yet been investigated in this high-risk group. Therefore, this study examined COX-2 mRNA and protein expression in oesophageal biopsies and resected tissues of 44 HNC patients. The evaluation covered 55 oesophageal tissue samples (18 invasive oesophageal squamous cell cancers, four high- and eight low-grade dysplasias, 25 normal squamous epithelia) from the 44 patients. mRNA levels of COX-2 were measured by real-time PCR using a LightCycler. COX-2 protein expression was studied immunohistochemically and graded by a staining score. COX-2 mRNA was detected in all samples, and its levels correlated positively with the immunohistochemical staining score (P |
| Databáze: | OpenAIRE |
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