UNC-80 and the NCA Ion Channels Contribute to Endocytosis Defects in Synaptojanin Mutants
| Autor: | Erik M. Jorgensen, Kevin S. Hamming, Sean D. Young, Terrance P. Snutch, Colin Thacker, Shigeki Watanabe, Kim Schuske, Maelle Jospin, Deepa Joshi |
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| Rok vydání: | 2007 |
| Předmět: |
Green Fluorescent Proteins
Mutant Phosphatase Nerve Tissue Proteins Synaptojanin Endocytosis Synaptic Transmission Synaptic vesicle Ion Channels MOLNEURO General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Animals Synaptic vesicle recycling Caenorhabditis elegans Caenorhabditis elegans Proteins Ion channel 030304 developmental biology Neurons 0303 health sciences Models Genetic Agricultural and Biological Sciences(all) biology Biochemistry Genetics and Molecular Biology(all) biology.organism_classification Axons Phosphoric Monoester Hydrolases Cell biology Mutation CELLBIO Synaptic Vesicles General Agricultural and Biological Sciences 030217 neurology & neurosurgery |
| Zdroj: | Current Biology. 17:1595-1600 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2007.08.036 |
| Popis: | SummarySynaptojanin is a lipid phosphatase required to degrade phosphatidylinositol 4,5 bisphosphate (PIP2) at cell membranes during synaptic vesicle recycling [1, 2]. Synaptojanin mutants in C. elegans are severely uncoordinated and are depleted of synaptic vesicles, possibly because of accumulation of PIP2[2]. To identify proteins that act downstream of PIP2 during endocytosis, we screened for suppressors of synaptojanin mutants in the nematode C. elegans. A class of uncoordinated mutants called “fainters” partially suppress the locomotory, vesicle depletion, and electrophysiological defects in synaptojanin mutants. These suppressor loci include the genes for the NCA ion channels [3], which are homologs of the vertebrate cation leak channel NALCN [4], and a novel gene called unc-80. We demonstrate that unc-80 encodes a novel, but highly conserved, neuronal protein required for the proper localization of the NCA-1 and NCA-2 ion channel subunits. These data suggest that activation of the NCA ion channel in synaptojanin mutants leads to defects in recycling of synaptic vesicles. |
| Databáze: | OpenAIRE |
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