Isolation and characterization of a transposon-induced cytotoxin-deficient mutant of Pseudomonas aeruginosa
Autor: | M C Hammer, A L Baltch, L H Bopp, R P Smith, M A Franke, F Lutz |
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Rok vydání: | 1991 |
Předmět: |
Transposable element
Immunology Mutant Mutagenesis (molecular biology technique) Biology medicine.disease_cause Microbiology fluids and secretions Kanamycin medicine Cytotoxins Pseudomonas aeruginosa Drug Resistance Microbial bacterial infections and mycoses biology.organism_classification digestive system diseases Infectious Diseases Mutation Pseudomonadales DNA Transposable Elements bacteria Parasitology Bacteria Research Article Pseudomonadaceae medicine.drug |
Zdroj: | Infection and Immunity. 59:708-711 |
ISSN: | 1098-5522 0019-9567 |
Popis: | In order to provide a better system for investigating the role of cytotoxin in pathogenesis, we mutated wild-type Pseudomonas aeruginosa PA158 by introducing a transposon. The resulting pool of mutants was screened for cytotoxin-deficient strains. One mutant strain, PA114F5, was compared with PA158. Except for cytotoxin production and antibiotic resistance (specified by the transposon), the two strains appear isogenic. This mutant strain should be useful in further clarifying the role of cytotoxin in pathogenesis. |
Databáze: | OpenAIRE |
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