A Jasmonate-Inducible Defense Trait Transferred from Wild into Cultivated Tomato Establishes Increased Whitefly Resistance and Reduced Viral Disease Incidence
| Autor: | Escobar-Bravo R, Jm, Alba, Clara Pons, Granell A, Kant MR, Moriones E, Fernández-Muñoz R |
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| Přispěvatelé: | Faculty of Science, IBED Other Research (FNWI), Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Netherlands Organization for Scientific Research, European Commission |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine acylsugars Whitefly Plant Science lcsh:Plant culture Methyl jasmonate 01 natural sciences Bemisia tabaci Plant breeding Tomato 03 medical and health sciences chemistry.chemical_compound Methyl Jasmonate Botany Tomato yellow leaf curl virus plant breeding whitefly Wild tomato lcsh:SB1-1110 Jasmonate Acylsugars Original Research 2. Zero hunger biology fungi food and beverages Trichomes biology.organism_classification Solanum pimpinellifolium Trichome 030104 developmental biology chemistry trichomes Solanum 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 7 (2016) RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname Frontiers in Plant Science Frontiers in Plant Science, 7:1732. Frontiers Media S.A. Europe PubMed Central Frontiers in Plant Science, 7, 1732 Digital.CSIC. Repositorio Institucional del CSIC |
| ISSN: | 1664-462X |
| Popis: | Whiteflies damage tomatoes mostly via the viruses they transmit. Cultivated tomatoes lack many of the resistances of their wild relatives. In order to increase protection to its major pest, the whitefly Bemisia tabaci and its transmitted Tomato Yellow Leaf Curl Virus (TYLCV), we introgressed a trichome-based resistance trait from the wild tomato Solanum pimpinellifolium into cultivated tomato, Solanum lycopersicum. The tomato backcross line BC5S2 contains acylsucrose-producing type-IV trichomes, unlike cultivated tomatoes, and exhibits increased, yet limited protection to whiteflies at early development stages. Treatment of young plants with methyl jasmonate (MeJA) resulted in a 60% increase in type-IV trichome density, acylsucrose production, and enhanced resistance to whiteflies, leading to 50% decrease in the virus disease incidence compared to cultivated tomato. Using transcriptomics, metabolite analysis, and insect bioassays we established the basis of this inducible resistance. We found that MeJA activated the expression of the genes involved in the biosynthesis of the defensive acylsugars in young BC5S2 plants leading to enhanced chemical defenses in their acquired type-IV trichomes. Our results show that not only constitutive but also these inducible defenses can be transferred from wild into cultivated crops to aid sustainable protection, suggesting that conventional breeding strategies provide a feasible alternative to increase pest resistance in tomato. This work was funded by grants AGL2013-48913-C2-01-R/AGR (Spanish MICINN, co-financed by EU FEDER) and P10-AGR-6516 (Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain). RE-B was recipient of a FPI grant by MICINN. JA was supported by the Netherlands Organization for Scientific Research NWO (Earth and Life Sciences ALW/TOP 854.11.005). MK was supported by Netherlands Organization for Scientific Research NWO (Technology Foundation STW/VIDI 13492). EM and RF-M are members of AGR-214 and AGR-129 research groups, respectively, partially funded by the Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain (co-financed by EU FEDER-FSE). AG was funded by TRADITOM (634561) under the H2020 SFS-07a-2014 EU Program, the Spanish Ministry BIO2013-42193-R, and the EU COST-FA1106. |
| Databáze: | OpenAIRE |
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