BoLA class I allele diversity and polymorphism in a herd of cattle
| Autor: | Reno Pontarollo, Chenhong Zhang, Shawn Babiuk, Mik Bickis, Benjamin Horseman, Anthony Kusalik, Mitchell S. Abrahamsen, Lawrence B. Schook |
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| Rok vydání: | 2007 |
| Předmět: |
Molecular Sequence Data
Immunology Population Genes MHC Class I Human leukocyte antigen Biology Major histocompatibility complex Negative selection Antigen MHC class I Genetic variation Genetics Animals Humans Amino Acid Sequence Cloning Molecular Selection Genetic Allele education Alleles Phylogeny Recombination Genetic education.field_of_study Polymorphism Genetic Sequence Homology Amino Acid Histocompatibility Antigens Class I Genetic Variation biology.protein Cattle |
| Zdroj: | Immunogenetics. 59:167-176 |
| ISSN: | 1432-1211 0093-7711 |
| DOI: | 10.1007/s00251-006-0173-7 |
| Popis: | Major histocompatibility complex class I genes are among the most polymorphic genes characterized. The high level of polymorphism is essential for generating host immune responses. In humans, three distinct genomic loci encode human leukocyte antigen (HLA) class I genes, allowing individuals to express up to six different HLA class I molecules. In cattle, the number of distinct genomic loci are currently at least six, and the number of different bovine leukocyte antigens (BoLA) class I molecules that are expressed in individual animals are variable. The extent of allele variation within the cattle population is unknown. In this study, the number and variety of BoLA class I sequences expressed by 36 individuals were determined from full-length BoLA class I cDNA clones. Twenty distinct BoLA class I alleles were identified, with only four being previously reported. The number of expressed BoLA class I alleles in individual animals ranged between one and four, with none of the animals having an identical complement of BoLA class I molecules. Variation existed in the number of BoLA class I alleles expressed as well as the composition of expressed alleles, however, several BoLA class I alleles were found in multiple individual animals. Polymorphic amino acid sites were analyzed for positive and negative selection using the ADAPTSITE program. In the antigen recognition sites (ARS), there were eight positions that were predicted to be under positive selection and three positions that were predicted to be under negative selection from 62 positions. In contrast, for non-antigen recognition sites (non-ARS), there were three positions that were predicted to be under positive selection and 20 that were predicted to be under negative selection from 278, indicating that positive selection of amino acids occurs at a greater frequency within the antigen recognition sites. |
| Databáze: | OpenAIRE |
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