DNA template-assisted modulation of horseradish peroxidase activity
| Autor: | Shohini Ghosh Datta, Xiaozheng Dou, Alyxandria Shibley, Bhaskar Datta |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Biochemistry
Horseradish peroxidase Substrate Specificity Enzyme catalysis chemistry.chemical_compound Ultraviolet visible spectroscopy Phenols Structural Biology Animals Phenol Organic chemistry Molecule Base Pairing Molecular Biology Horseradish Peroxidase chemistry.chemical_classification biology DNA General Medicine Combinatorial chemistry Ampyrone Enzyme chemistry Yield (chemistry) biology.protein Cattle Oxidation-Reduction |
| Zdroj: | International Journal of Biological Macromolecules. 50:552-557 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2012.01.028 |
| Popis: | The precise alignment of DNA molecules by Watson–Crick base-pairing combined with its polymeric characteristics have allowed DNA to be used as a template or scaffold for assembling materials. In this work, we investigate the role of calf-thymus DNA as a template for enhancing the horseradish peroxidase (HRP)-mediated oxidation of phenol and phenolic derivatives. The HRP-catalyzed oxidation of phenol into polyphenolic products and in presence of 4-aminoantipyrine into quinoneimine dye complexes is studied. Visible spectroscopy reveals an increased yield of both products of the enzymatic reaction in presence of calf-thymus DNA and is attributed to the prearrangement of the corresponding substrates on the DNA. The concentrations of calf-thymus DNA and the substrates are found to affect the nature of prearrangement and subsequent formation of polymeric or co-oxidation products. Also, phenolic derivatives with different aromatic substitutions display divergent propensities towards product formation in presence of the DNA template. Our results demonstrate the ability of calf-thymus DNA to modulate the activity of HRP and exercise control on the nature of products formed. This work highlights the potential of using DNA as a template for influencing enzymatic reactions involving aromatic substrates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect