Anesthetic regimen for cardiac function evaluation by echocardiography in mice: comparison between ketamine, etomidate and isoflurane versus conscious state
| Autor: | Pauline Marchal, Jeanne Mialet-Perez, Vincent Minville, Denis Calise, Stéphanie Ruiz, Laurent Lonjaret, Nicolas Franchitto, Angelo Parini, Olivier Lairez, Olivier Fourcade |
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| Přispěvatelé: | Centre d'Imagerie Cardiaque, CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de cardiologie [Toulouse], Hôpital de Rangueil, CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse], Service d'Anesthésie-Réanimation, CHU Toulouse [Toulouse]-Institut Louis Bugnard-Hôpital de Rangueil, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Service Cardiologie [CHU Toulouse], Pôle Cardiovasculaire et Métabolique [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Pôle Anesthésie Réanimation [CHU de Toulouse], Simon, Marie Francoise |
| Rok vydání: | 2013 |
| Předmět: |
Cardiac function curve
Cardiac output MESH: Etomidate Diastole MESH: Anesthetics 030204 cardiovascular system & hematology MESH: Ventricular Function Left Ventricular Function Left 03 medical and health sciences Mice 0302 clinical medicine Etomidate Heart Rate Heart rate medicine Animals Ketamine MESH: Animals MESH: Heart Rate Cardiac Output MESH: Isoflurane MESH: Mice 030304 developmental biology Anesthetics 0303 health sciences General Veterinary Isoflurane business.industry MESH: Cardiac Output MESH: Ketamine Heart 3. Good health MESH: Heart Echocardiography Anesthesia Anesthetic Animal Science and Zoology MESH: Echocardiography business medicine.drug |
| Zdroj: | Laboratory Animals Laboratory Animals, SAGE Publications, 2013, 47 (4), pp.284-90. ⟨10.1177/0023677213496236⟩ Laboratory Animals, 2013, 47 (4), pp.284-90. ⟨10.1177/0023677213496236⟩ |
| ISSN: | 1758-1117 0023-6772 |
| DOI: | 10.1177/0023677213496236⟩ |
| Popis: | International audience; Mice with genetic alterations are used in heart research for the extrapolation of human diseases. Echocardiography is an essential tool for evaluating cardiac and hemodynamic functions in small animals. The purpose of this study was to compare the effect of different anesthetic regimens and the conscious state on the evaluation of cardiac function by echocardiography. Mice were examined in the conscious state after three days of training, and then for a 7 min period after a single intraperitoneal injection of ketamine at 100 mg/kg, etomidate at 10, 20 or 30 mg/kg, or after inhalation of isoflurane at 1.5% with or without a short period of induction with isoflurane 3%. Intra- and inter-observer variabilities were assessed. The operator's comfort was also assessed. Heart rate, left ventricular end diastolic diameter, fraction shortening and cardiac output were measured using echocardiography. Ketamine at 5 and 7 min after induction and isoflurane at 3, 5 and 7 min after induction provided good anesthetic conditions and a quick awakening time, and did not influence cardiac performance, whereas the conscious state was associated with a non-physiological sympathetic activation and other anesthetic drugs induced a significant decrease in heart rate. Etomidate 10 mg/kg and 20 mg/kg were not enough to provide adequate anesthesia. Etomidate 30 mg/kg induced a good anesthetic condition but influenced cardiac performance and had a long awakening time. Our results indicate that ketamine and isoflurane with a short induction period are better anesthetic drugs than isoflurane without induction or etomidate for evaluating cardiac function in healthy mice. |
| Databáze: | OpenAIRE |
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