Localization of antibody binding sites in the larvae of Ascaris lumbricoides var. suum by means of fluorescent technics
| Autor: | Rene A. Echevarria, Catherine A. Crandall, Victor M. Arean |
|---|---|
| Rok vydání: | 1963 |
| Předmět: |
animal structures
Immunology Fluorescent Antibody Technique Antibodies parasitic diseases Animals Helminths Ascaris lumbricoides Coloring Agents Cuticle (hair) Ascariasis biology Ascaris Research fungi Embryonated General Medicine Anatomy biology.organism_classification Molecular biology Staining Infectious Diseases Excretory system Larva biology.protein Parasitology Binding Sites Antibody Rabbits Antibody |
| Zdroj: | Experimental Parasitology. 14:296-303 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/0014-4894(63)90034-1 |
| Popis: | Antibody binding sites in Ascaris lumbricoides var. suum were determined by direct and indirect fluorescent antibody technics. Binding sites on embryonated eggs, freshly hatched second stage larvae, and larvae isolated from and within the lungs and livers of experimentally infected mice, as well as sections of adult worms were investigated. Specific fluorescent staining was observed in oral, anal, cuticular, and excretory pore precipitates of larvae isolated from tissues and in the cuticle of whole and sectioned larvae. No precipitates were observed on second stage larvae; however, the cuticle stained specifically in both whole and sectioned second stage larvae when incubated in immune fluorescent globulin. Similarly, specific staining of the larval cuticle was noted in sections of embryonated eggs. The “fertilization” membrane of the latter stained nonspecifically. No staining of the cuticle in sections of adult worms was observed; only the periphery of the muscle cells showed specific staining. |
| Databáze: | OpenAIRE |
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