Forskolin induces the reorganization of extracellular matrix fibronectin and cytoarchitecture in 3T3-F442A adipocytes: its effect on fibronectin gene expression
| Autor: | Françoise Lasnier, Jocelyne Antras-Ferry, Françoise Hilliou, Jacques Pairault |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Forskolin
biology Colforsin 3T3 Cells Cell Biology Actin cytoskeleton Molecular biology FNDC5 Actins Fibronectins Fibronectin Extracellular matrix Mice chemistry.chemical_compound Adipose Tissue Gene Expression Regulation chemistry Adipogenesis Gene expression biology.protein Animals RNA Messenger Molecular Biology Actin |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1222:390-394 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/0167-4889(94)90045-0 |
| Popis: | We studied the effect of forskolin on fibronectin and actin gene expression in 3T3-F442A adipogenic cell line. The structural organizations of extracellular matrix fibronectin and actin cytoskeleton were investigated in parallel. Immunofluorescence experiments showed that preadipocytes treated for 48 h with 10 microM forskolin exhibited an intensified network of both actin and fibronectin when compared to control. A similar picture was obtained with adipocytes given long-term exposure to forskolin. As determined by Western analysis, fibronectin protein levels were increased by 50-75% over control, both in preadipocytes and adipocytes. A parallel increase of fibronectin mRNA content was observed in forskolin-treated cells. In contrast, forskolin treatment of preadipocytes and adipocytes did not elicit any change in the steady-state level of either actin mRNA or protein. Nuclear run-on experiments showed that forskolin increased the fibronectin gene transcription rate but left that of the actin gene in adipocytes unchanged. These findings suggest the reorganization of the actin network in forskolin-treated adipocytes to be a consequence of fibronectin-enhanced biosynthesis and reorganization. |
| Databáze: | OpenAIRE |
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