Comparison of PCR, capture ELISA and immunoblotting for detection of Toxoplasma gondii in infected mice
| Autor: | P. Flori, H. Raberin, J. Hafid, R. Tran Manh Sung |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Time Factors Ratón Immunoblotting Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Apicomplexa Mice law medicine Animals Parasite hosting Polymerase chain reaction biology Inoculation Reproducibility of Results Toxoplasma gondii General Medicine DNA Protozoan medicine.disease biology.organism_classification Virology Molecular biology Toxoplasmosis Toxoplasmosis Animal Protozoa Female Toxoplasma Injections Intraperitoneal |
| Zdroj: | Journal of Medical Microbiology. 50:1100-1104 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/0022-1317-50-12-1100 |
| Popis: | PCR was compared with capture ELISA and immunoblotting for the detection of Toxoplasma gondii in sera of acutely infected mice. One hundred animals were inoculated intraperitoneally with 5000 trophozoites of RH strain and five of them were killed every 3 h from 3 h to 21 h post infection (p.i.), and every day from day 1 to day 7 p.i.. No assay detected the parasite from 3 h p.i. to 15 h p.i. PCR was the most sensitive assay and detected the T. gondii from 18 h p.i., whereas the other assays detected it only from day 1. |
| Databáze: | OpenAIRE |
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