Construction of a modified mini-Tn5 luxCDABE transposon for the development of bacterial biosensors for ecotoxicity testing
| Autor: | L. Anne Glover, David A. Bailey, Ken Killham, Jenny M Ritchie, Alison Margaret Horsburgh, Hedda J. Weitz |
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| Rok vydání: | 2001 |
| Předmět: |
Pseudomonas fluorescens
Biosensing Techniques Microbiology Pseudomonas Toxicity Tests Genetics Bioluminescence Cloning Molecular Molecular Biology Vibrio Bacteria biology Transposon integration Water biology.organism_classification Pseudomonas putida Zinc Conjugation Genetic Luminescent Measurements Pseudomonadales DNA Transposable Elements bacteria Bioreporter Copper Chlorophenols Pseudomonadaceae |
| Zdroj: | FEMS Microbiology Letters. 197:159-165 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2001.tb10598.x |
| Popis: | A mini-Tn5 transposon was modified to introduce a promoterless luxCDABE cassette from Vibrio fischeri into environmentally relevant bacterial strains in order to develop bioluminescence-based biosensors for toxicity testing. The mini-Tn5 luxCDABE transposon was chromosomally integrated downstream from an active promoter into two Pseudomonas strains (Pseudomonas fluorescens 8866 and Pseudomonas putida F1). Characterisation of the bioluminescent transconjugants demonstrated that the transposon integration was stable and had no effect on growth rate. Both P. fluorescens 8866 Tn5 luxCDABE and P. putida F1 Tn5 luxCDABE were used to assess the toxicity of standard solutions (Cu, Zn and 3,5-DCP) as well as Cu- and 3,5-DCP-spiked groundwater samples. They were successfully used for bioluminescence-based bioassays and the potential value of using different bacterial biosensors for ecotoxicity testing was shown. |
| Databáze: | OpenAIRE |
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