Engineering transferrable microvascular meshes for subcutaneous islet transplantation
| Autor: | Robert E. Schwartz, Vivian K. Lee, James A. Flanders, Bin Li, Alan Chiu, Minglin Ma, Daniel T. Bowers, Guohao Dai, Xi Wang, Duo An, Yehudah Pardo, Long-Hai Wang, Nikolaos Bouklas, Wei Song, Qingsheng Liu, Soon Hon Cheong |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Science Induced Pluripotent Stem Cells Cell Culture Techniques Islets of Langerhans Transplantation General Physics and Astronomy Neovascularization Physiologic Bioengineering 02 engineering and technology Mice SCID General Biochemistry Genetics and Molecular Biology Article Diabetes Mellitus Experimental Neovascularization Rats Sprague-Dawley 03 medical and health sciences Experimental therapy medicine Human Umbilical Vein Endothelial Cells Animals Humans Tissue engineering lcsh:Science Induced pluripotent stem cell 030304 developmental biology 0303 health sciences geography Multidisciplinary geography.geographical_feature_category business.industry Extramural Regeneration (biology) General Chemistry 021001 nanoscience & nanotechnology Islet Sprague dawley Transplantation Type 1 diabetes Hyperglycemia Microvessels lcsh:Q Female medicine.symptom 0210 nano-technology business Biomedical engineering |
| Zdroj: | Nature Communications Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2041-1723 |
| Popis: | The success of engineered cell or tissue implants is dependent on vascular regeneration to meet adequate metabolic requirements. However, development of a broadly applicable strategy for stable and functional vascularization has remained challenging. We report here highly organized and resilient microvascular meshes fabricated through a controllable anchored self-assembly method. The microvascular meshes are scalable to centimeters, almost free of defects and transferrable to diverse substrates, ready for transplantation. They promote formation of functional blood vessels, with a density as high as ~220 vessels mm-2, in the poorly vascularized subcutaneous space of SCID-Beige mice. We further demonstrate the feasibility of fabricating microvascular meshes from human induced pluripotent stem cell-derived endothelial cells, opening a way to engineer patient-specific microvasculature. As a proof-of-concept for type 1 diabetes treatment, we combine microvascular meshes and subcutaneously transplanted rat islets and achieve correction of chemically induced diabetes in SCID-Beige mice for 3 months. The success of engineered tissue depends on the integration of a dense vascular network to supply nutrients and remove waste products. Here the authors design high density microvascular meshes made through an anchored self-assembly mechanism, and use these meshes to support subcutaneous pancreatic islet survival in a mouse diabetes model. |
| Databáze: | OpenAIRE |
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