Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
| Autor: | Kanwaldeep Dhaliwal, Ahsan-Ul-Haq Akram, Mark Bradley, Bethany Mills, Emma Scholefield |
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| Rok vydání: | 2017 |
| Předmět: |
Confocal laser endomicroscopy
General Immunology and Microbiology biology business.industry General Chemical Engineering General Neuroscience Confocal biology.organism_classification General Biochemistry Genetics and Molecular Biology Human lung 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure 030228 respiratory system 030220 oncology & carcinogenesis Microscopy medicine Endomicroscopy Fluorescence microscope business Ex vivo Bacteria Biomedical engineering |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| Popis: | Improving the speed and accuracy of bacterial detection is important for patient stratification and to ensure the appropriate use of antimicrobials. To achieve this goal, the development of diagnostic techniques to recognize bacterial presence in real-time at the point-of-care is required. Optical imaging for direct identification of bacteria within the host is an attractive approach. Several attempts at chemical probe design and validation have been investigated, however none have yet been successfully translated into the clinic. Here we describe a method for ex vivo validation of bacteria-specific probes for identification of bacteria within the distal lung, imaged by fibered confocal fluorescence microscopy (FCFM). Our model used ex vivo human lung tissue and a clinically approved confocal laser endomicroscopy (CLE) platform to screen novel bacteria-specific imaging compounds, closely mimicking imaging conditions expected to be encountered with patients. Therefore, screening compounds by this technique provides confidence of potential clinical tractability. |
| Databáze: | OpenAIRE |
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