Silymarin protects spinal cord and cortical cells against oxidative stress and lipopolysaccharide stimulation
| Autor: | Di You Lin, Henrich Cheng, May Jywan Tsai, Chin Wen Chi, Jyh Fei Liao, Wen Cheng Huang, Ming Chao Huang, Hsin Chun Yang, Dann Ying Liou, Hsin Jung Lee, Ya Tzu Chen |
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| Rok vydání: | 2009 |
| Předmět: |
Lipopolysaccharides
Central nervous system Silibinin Pharmacology medicine.disease_cause Neuroprotection Glial cell proliferation Antioxidants Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound Medicine Animals Cell damage Spinal cord injury Cells Cultured Spinal Cord Injuries Cerebral Cortex business.industry Cell Biology medicine.disease Coculture Techniques Rats Disease Models Animal Oxidative Stress medicine.anatomical_structure Neuroprotective Agents chemistry Spinal Cord Brain Injuries Immunology Neuroglia business Oxidative stress Silymarin |
| Zdroj: | Neurochemistry international. 57(8) |
| ISSN: | 1872-9754 |
| Popis: | Contusive spinal cord injury (SCI) is a devastating event which leads to a loss of neurological function below the level of injury. A secondary degenerative process is initiated following acute SCI. This secondary cascade provides opportunities for the delivery of therapeutic interventions. Silymarin, a widely used “liver herb”, is frequently used for the protection against various hepatobiliary problems. However, the effectiveness of silymarin in central nervous system (CNS), especially in spinal cord, is not firmly established. The present work evaluates the effects of silymarin and its major constituent, silybin, on oxidative stress and lipopolysaccharide (LPS) stimulation in primary neuronal/glial cell cultures and in vivo . Silymarin or silybin inhibited glial cell proliferation in a concentration-dependent manner. Furthermore, it protected glial cells against peroxide-induced reactive oxygen species (ROS) formation, ATP depletion, and cell damage. Interestingly, the inhibition of peroxide-induced ROS by silybin could be partially attenuated by inhibitors of NFκB or protein kinase C (PKC), suggesting an involvement of NFκB and PKC signaling pathways. In mixed neuronal/glial cell cultures from cerebral cortex or spinal cord, silymarin or silybin effectively attenuated peroxide-induced ROS formation, with silymarin being more effective than silybin, implicating other constituents of silymarin that may be involved. Consistently, silymarin reduced LPS-induced injures in spinal neuronal/glial cell cultures. In vivo , intrathecal administration of silymarin immediately after eliciting contusive SCI effectively improved hindlimb locomotor behavior in the rats. Taken together, silymarin or silybin shows promise in protecting the CNS cells from toxin- or injury-induced damages and might be used to treat head- or spinal cord-injuries related to free radical assault. |
| Databáze: | OpenAIRE |
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