Nucleic acid-induced potentiation of matrix metalloproteinase-9 enzymatic activity
| Autor: | Tyler Duellman, Rie Wakamiya, Jay Yang, Xi Chen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Protein Conformation Neuronal Outgrowth enzymatic activity MMP9 Matrix metalloproteinase Biochemistry PC12 Cells Gene Expression Regulation Enzymologic 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Image Processing Computer-Assisted Animals Humans Molecular Biology Research Articles chemistry.chemical_classification Chemistry RNA Biological activity Cell Biology Rats body regions modulation nucleic acids 030104 developmental biology Enzyme HEK293 Cells Matrix Metalloproteinase 9 Polynucleotide Nucleic acid pharmacology 030217 neurology & neurosurgery DNA Research Article |
| Zdroj: | Biochemical Journal |
| ISSN: | 1470-8728 |
| Popis: | Matrix metalloproteinases (MMPs) play varied roles in normal biology and diseases where, depending on the context, both inhibition and enhancement of the enzymatic activity may be beneficial. However, there are very few reports of positive modulators of MMP activity. We report that polynucleotides, including single-stranded DNA, RNA, and even double-stranded DNA, bind to and enhance the enzymatic activity of MMP9. This enhancement of MMP9 catalytic activity is not shared by biologically active polycationic molecules suggesting nonspecific charge screening as an unlikely mechanism. Deletion construct and MMP1, 2, and 3 studies suggest that the type-II fibronectin repeat domains of the enzyme appear to play a role in mediating the nucleotide potentiation of MMP9 activity. Single-stranded DNA enhances nerve growth factor-induced MMP9-dependent neurite extension in pheochromocytoma 12 cells providing evidence for potential biological significance of the nucleotide-mediated allosteric enhancement of the catalytic activity. |
| Databáze: | OpenAIRE |
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