Ghrelin inhibit cell apoptosis in pancreatic β cell line HIT-T15 via mitogen-activated protein kinase/phosphoinositide 3-kinase pathways
| Autor: | Hong Fan, Dai-Shun Liu, Yonggang Zhang, Yuwei Zhang, Yonggang Wei, Dan Xu, Binwu Ying, Tao Wang, Xiaohong Zhang, Liyang Dou, Dongmei Yang, LiXin Shi, Fei Jiang, Fuqiang Wen, Guo Chen, Xiaobo Hu |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
MAPK/ERK pathway
Programmed cell death medicine.medical_specialty Time Factors Cell Survival Peptide Hormones Apoptosis Toxicology Cell Line Phosphatidylinositol 3-Kinases Cricetinae Insulin-Secreting Cells Internal medicine In Situ Nick-End Labeling medicine Animals Enzyme Inhibitors Phosphorylation Protein kinase B PI3K/AKT/mTOR pathway Phosphoinositide-3 Kinase Inhibitors Phosphoinositide 3-kinase biology digestive oral and skin physiology Ghrelin Cell biology Endocrinology Doxorubicin Mitogen-activated protein kinase biology.protein Mitogen-Activated Protein Kinases |
| Zdroj: | Toxicology. 237:194-202 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/j.tox.2007.05.013 |
| Popis: | Ghrelin stimulates growth hormone (GH) release and induces positive energy balances. Previous studies have reported that ghrelin inhibits apoptosis in several cell types but the precise underlying protective mechanisms in pancreatic beta cells are poorly understood. Therefore, we investigated which pathway was related with its anti-apoptotic effect in pancreatic beta cells. Exposure of HIT-T15 cells to ghrelin caused a rapid activation of MAPKs and Akt. Chemical inhibitors of MAPK and PI3K blocked the anti-apoptotic of ghrelin. Ghrelin also stimulated the mitochondrial pathways of apoptosis and it showed increased Bcl-2, decreased Bax, prevention cytochrome c release and inhibition of caspase-3 activation in pancreatic beta cell line HIT-T15. Our findings suggest that ghrelin may act as a survival factor that inhibits the apoptotics pathways, and the MAPKs, AKT pathways could be key roles in the apoptosis of pancreatic beta cells. |
| Databáze: | OpenAIRE |
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