Dermatophagoides pteronyssinus 2 regulates nerve growth factor release to induce airway inflammation via a reactive oxygen species-dependent pathway
| Autor: | Yi Ling Ye, Chiou Feng Lin, Hung Tsung Wu, Chia Yuan Hsieh, Jiu Yao Wang, Yu Lun Cheng, Fu Hua Liu, Ching Chun Chen, Ching Ting Ma, Bor-Luen Chiang, Chia Hao Bei, Chiung Wen Tsao |
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| Rok vydání: | 2011 |
| Předmět: |
Pulmonary and Respiratory Medicine
MAP Kinase Signaling System Physiology medicine.medical_treatment Inflammation Biology medicine.disease_cause Arthropod Proteins Cell Line Mice Allergen Physiology (medical) Nerve Growth Factor medicine Animals Antigens Dermatophagoides Respiratory system Lung chemistry.chemical_classification Reactive oxygen species Protease Epithelial Cells Cell Biology Allergens respiratory system Asthma respiratory tract diseases Mice Inbred C57BL Disease Models Animal Nerve growth factor chemistry Mitogen-activated protein kinase Immunology 1 2-Dihydroxybenzene-3 5-Disulfonic Acid Disodium Salt biology.protein Liberation Female medicine.symptom Reactive Oxygen Species Bronchoalveolar Lavage Fluid |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 300:L216-L224 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.00165.2010 |
| Popis: | Group 2 allergen of Dermatophagoides pteronyssinus 2 (Der p2) induces airway inflammation without protease activity, and elevated nerve growth factor (NGF) levels are also found in this inflammation. How the allergen Der p2 regulates NGF release via reactive oxygen species (ROS) to induce inflammation remains unclear. In the present study, intratracheal administration of Der p2 to mice led to inflammatory cell infiltration, mucus gland hyperplasia, and NGF upregulation in the bronchial epithelium, as well as elevated ROS and NGF production in bronchoalveolar lavage fluids. In addition, Der p2 caused fibrocyte accumulation and mild fibrosis. p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) inhibitors inhibited Der p2-induced NGF release in LA4 lung epithelial cells and MLg lung fibroblasts. Pretreatment with an antioxidant, tiron, reduced the Der p2-induced ROS production, NGF expression and release, p38 MAPK or JNK phosphorylation, and airway inflammation. These results suggest that Der p2 allergen-induced airway inflammation and elevated NGF release were through increasing ROS production and a MAPK-dependent pathway. The use of an antioxidant, tiron, may provide a new therapeutic modality for the treatment of allergic asthma. |
| Databáze: | OpenAIRE |
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