Characterization of a xenograft model for anti-CD19 CAR T cell studies
| Autor: | Sajjad Aghayan, Z Darvishyan, Samad Muhammadnejad, Mansour Jamali, Kobra Moradzadeh, S Alatab, Naser Ahmadbeigi, Mohammad Vasei, A Ranjbar, A Khorsand |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Cancer Research T-Lymphocytes T cell Antigens CD19 Cell Immunotherapy Adoptive Mice Random Allocation 03 medical and health sciences 0302 clinical medicine Mice Inbred NOD Cell Line Tumor medicine Animals Neoplasm Invasiveness B cell Receptors Chimeric Antigen business.industry Lymphoma Non-Hodgkin Body Weight General Medicine medicine.disease Xenograft Model Antitumor Assays Chimeric antigen receptor Raji cell Lymphoma Disease Models Animal 030104 developmental biology medicine.anatomical_structure Lymphatic system Oncology 030220 oncology & carcinogenesis Cancer research Female Car t cells business |
| Zdroj: | Clinical and Translational Oncology. 23:2181-2190 |
| ISSN: | 1699-3055 1699-048X |
| DOI: | 10.1007/s12094-021-02626-5 |
| Popis: | Chimeric antigen receptor (CAR) T cell development for B cell malignancies treatment has triggered a paradigm shift in oncology. The development of anti-CD19 CAR T cells relies primarily on a panel of cell line-derived xenograft models, including Raji cells; however, the behavior of this model is under debate. We attempted to characterize this lymphoma model and propose outcome measures for CAR T cell studies Raji cell line was inoculated into NOG mice via intra-venous (IV), intra-peritoneal (IP), and subcutaneous (SC) routes with different inoculum sizes, and consequent clinical and histopathological outcomes were assessed. Inoculum sizes of 105–106 resulted in a complete take rate. The mice with IV and SC-inoculated Raji cells presented the shortest and longest survival among lymphoma-bearing mice, respectively (P |
| Databáze: | OpenAIRE |
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