Marked increase in ribosomal RNA gene multiplicity in a rat hepatoma cell line
| Autor: | Orlando J. Miller, Loh-Chung Yu, Paul Szabo, Dorothy A. Miller, Wolf Prensky, Ramana V. Tantravahi |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
DNA Replication
RNA Nucleic Acid Hybridization In situ hybridization DNA Biology Ribosomal RNA Molecular biology Chromosomes Chromosome Banding Rats chemistry.chemical_compound Liver Neoplasms Experimental chemistry Genes RNA Ribosomal 28S ribosomal RNA Karyotyping Genetics RNA polymerase I Animals Metaphase Gene Genetics (clinical) |
| Zdroj: | Chromosoma. 71(2) |
| ISSN: | 0009-5915 |
| Popis: | An H4-IIE-C3 hepatoma cell line derived from an ACI rat has been shown to have differentially stained regions attached to the short arms of chromosomes 3, 11 and 13 and the long arm of an unidentified small chromosome. There is cell to cell variability in the number and size of the differentially stained regions, which contain, on the average, about 5% of the total DNA. A series of secondary constrictions occur at intervals along the length of each differentially stained region. These stain with silver by the Ag-AS method, indicating that the differentially stained regions contain sites of active 45S ribosomal precursor RNA transcription. In situ hybridization to metaphase chromosomes shows that the hepatoma cells have a 10 fold increase in DNA coding for 18S and 28S ribosomal RNA, 90% of it located in the differentially stained regions, and no change in the number of genes coding for 5S RNA. These results have been confirmed by filter disc hybridization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink