Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry
| Autor: | Gerritsen, M G, Brinkman, P, Escobar Salazar, Natalia, Bos, L D, de Heer, K, Meijer, M, Janssen, H-G, de Cock, H, Wösten, H A B, Visser, C.E., van Oers, M H J, Sterk, P J, Sub Molecular Microbiology, Molecular Microbiology |
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| Přispěvatelé: | Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Food and Indoor Mycology, Graduate School, Pulmonology, Other departments, Medical Microbiology and Infection Prevention, ARD - Amsterdam Reproduction and Development, ACS - Heart failure & arrhythmias, Supramolecular Separations (HIMS, FNWI), Sub Molecular Microbiology, Molecular Microbiology |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030106 microbiology Conidiation Mass spectrometry Aspergillosis Gas Chromatography-Mass Spectrometry 03 medical and health sciences In vivo Taverne medicine Humans Invasive Pulmonary Aspergillosis Aspergillus Volatile Organic Compounds Chromatography biology Chemistry invasive pulmonary aspergillosis exhaled breath analysis General Medicine Invasive pulmonary aspergillosis biology.organism_classification medicine.disease gas chromatography-mass spectrometry 030104 developmental biology Infectious Diseases Breath Tests headspace analysis Gas chromatography Gas chromatography–mass spectrometry |
| Zdroj: | Medical Mycology, 56(2). Informa Healthcare Medical mycology, 56(2), 253-256. Informa Healthcare Medical Mycology, 56(2), 253-256. Informa Healthcare Medical Mycology, 56(2). Oxford University Press |
| ISSN: | 1460-2709 1369-3786 |
| Popis: | Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography–mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro. Each Aspergillus isolate produced a distinct VOC profile. These profiles could not be confirmed in exhaled breath in vivo. |
| Databáze: | OpenAIRE |
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