Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats

Autor: Ayca Tas, Z Soyer Sarica, Korhan Arslan, Muhammet Yasin Tekeli, Yavuz Silig, Murat Kanbur, Gökhan Eraslan, Mürsel Karabacak
Přispěvatelé: [Eraslan, G. -- Kanbur, M. -- Tekeli, M. Y.] Erciyes Univ, Dept Pharmacol & Toxicol, Fac Vet Med, TR-38090 Kayseri, Turkey -- [Karabacak, M.] Erciyes Univ, Dept Anim Hlth, Safiye Cikrikcioglu Vocat Collage, Kayseri, Turkey -- [Arslan, K.] Erciyes Univ, Dept Genet, Fac Vet Med, Kayseri, Turkey -- [Silig, Y.] Cumhuriyet Univ, Dept Med Biochem, Fac Med, Sivas, Turkey -- [Soyer Sarica, Z.] Erciyes Univ, Expt Res & Applicat Ctr, Kayseri, Turkey -- [Tas, A.] Cumhuriyet Univ, Dept Nutr & Diet, Fac Hlth Sci, Sivas, Turkey
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Rhododendron
Time Factors
Antioxidant
Health
Toxicology and Mutagenesis
medicine.medical_treatment
hepatic chemical metabolizing parameters
Nicotinamide adenine dinucleotide
Toxicology
medicine.disease_cause
Antioxidants
Nitric oxide
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
medicine
oxidative stress
Animals
rat
Rats
Wistar
grayanotoxin
Micronuclei
Chromosome-Defective
genotoxic effect
Micronucleus Tests
biology
Honey
General Medicine
Glutathione
Malondialdehyde
Mad honey
Oxidative Stress
030104 developmental biology
Endocrinology
Liver
chemistry
biology.protein
Comet Assay
Diterpenes
Biomarkers
030217 neurology & neurosurgery
Nicotinamide adenine dinucleotide phosphate
Oxidative stress
DNA Damage
Zdroj: Human & Experimental Toxicology. 37:991-1004
ISSN: 1477-0903
0960-3271
Popis: WOS: 000441925600013
PubMed ID: 29271245
A total of 66 male Wistar rats were used and six groups (control: 10 animals and experimental: 12 animals) were formed. While a separate control group was established for each study period, mad honey application to the animals in the experimental group was carried out with a single dose (12.5 g kg(-1) body weight (b.w.); acute stage), at a dose of 7.5 g kg(-1) b.w. for 21 days (subacute stage), and at a dose of 5 g kg(-1) b.w. for 60 days (chronic stage). Tissue and blood oxidative stress markers (malondialdehyde (MDA), nitric oxide (NO), 4-hydroxynonenal (HNE), superoxide dismutase, catalase, glutathione (GSH) peroxidase, and glucose-6-phosphate dehydrogenase), hepatic chemical metabolizing parameters in the liver (cytochrome P450 2E1, nicotinamide adenine dinucleotide (NADH)-cytochrome b5 reductase, nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome c reductase (CYTC), GSH S-transferase (GST), and GSH), and micronucleus and comet test in some samples were examined. Findings from the study showed that single and repeated doses given over the period increased MDA, NO, and HNE levels while decreasing/increasing tissue and blood antioxidant enzyme activities. From hepatic chemical metabolizing parameters, GST activity increased in the subacute and chronic stages and CYTC activity increased in the acute period, whereas GSH level decreased in the subacute stage. Changes in tail and head intensities were found in most of the comet results. Mad honey caused oxidative stresses for each exposure period and made some significant changes on the comet test in certain periods for some samples obtained. In other words, according to the available research results obtained, careless consumption of mad honey for different medical purposes is not appropriate.
Databáze: OpenAIRE
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