Target molecules in 3T3-L1 adipocytes differentiation are regulated by maslinic acid, a natural triterpene from Olea europaea
| Autor: | Eva E. Rufino-Palomares, Leticia García-Salguero, Pedro P. Medina, Nieves Fernández-Gallego, Amalia Pérez-Jiménez, M. Carmen Ortuño-Costela, Juan Peragón, Fernando J. Reyes-Zurita, José A. Lupiáñez, Khalida Mokhtari |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Survival Glucose uptake Pharmaceutical Science Biology Fatty Acid-Binding Proteins Mice 03 medical and health sciences chemistry.chemical_compound Maslinic acid 3T3-L1 Cells Olea Adipocyte Drug Discovery Adipocytes Animals Viability assay Triglycerides Pharmacology Adipogenesis Cell Differentiation 3T3-L1 Peroxisome Triterpenes PPAR gamma Glucose 030104 developmental biology Complementary and alternative medicine chemistry Biochemistry RNA Molecular Medicine Calcium Intracellular |
| Zdroj: | Phytomedicine. 23:1301-1311 |
| ISSN: | 0944-7113 |
| DOI: | 10.1016/j.phymed.2016.07.001 |
| Popis: | Background Metabolic syndrome is a set of pathologies among which stand out the obesity, which is related to the lipid droplet accumulation and changes to cellular morphology regulated by several molecules and transcription factors. Maslinic acid (MA) is a natural product with demonstrated pharmacological functions including anti-inflammation, anti-tumor and anti-oxidation, among others. Purpose Here we report the effects of MA on the adipogenesis process in 3T3-L1 cells. Methods Cell viability, glucose uptake, cytoplasmic triglyceride droplets, triglycerides quantification, gene transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ) and adipocyte fatty acid-binding protein (aP2) and intracellular Ca2+ levels were determined in pre-adipocytes and adipocytes of 3T3-L1 cells. Results MA increased glucose uptake. MA also decreased lipid droplets and triglyceride levels, which is in concordance with the down-regulation of PPARγ and aP2. Finally, MA increased the intracellular Ca2+ concentration, which could also be involved in the demonstrated antiadipogenic effect of this triterpene. Conclusion MA has been demonstrated as potential antiadipogenic compound in 3T3-L1 cells |
| Databáze: | OpenAIRE |
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