IL-15 Expression at Human Endometrium and Decidua
| Autor: | Izumi Yagi, Jinsuke Yasuda, Hideo Honjo, Kotaro Kitaya, Y. Tada, Shinji Fushiki |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Stromal cell medicine.medical_treatment Gene Expression Biology Endometrium Natural killer cell Andrology Pregnancy Internal medicine Decidua medicine Humans Decidual cells RNA Messenger Cells Cultured Menstrual Cycle Interleukin-15 Mucous Membrane Reverse Transcriptase Polymerase Chain Reaction Uterus Interleukin Cell Biology General Medicine Immunohistochemistry Killer Cells Natural Pregnancy Trimester First Endocrinology medicine.anatomical_structure Cytokine Reproductive Medicine Interleukin 15 Female |
| Zdroj: | Biology of Reproduction. 63:683-687 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod63.3.683 |
| Popis: | A large number of natural killer (NK) cells appear in human uterine mucosa during the secretory phase and first trimester pregnancy. We investigated the expression of interleukin (IL)-15, a possible stimulator for these NK cells, in human endometrium and first trimester decidua. Semiquantitative reverse transcriptase-polymerase chain reaction revealed that IL-15 mRNA expression was stronger during the secretory phase and first trimester pregnancy than during the proliferative phase. Immunohistochemistry revealed that immunoreactivity for anti-IL-15 was higher during the secretory phase than it was during the proliferative phase. This was prominent in the perivascular stromal cells around invading spiral arteries during the mid- to late-secretory phase. In first trimester decidua, endothelial cells were also stained as strongly as stromal cells. A membrane-bound IL-15 molecule was detected on the surface of first trimester decidual cells by flow cytometry. Progesterone stimulated the release of soluble IL-15 in the supernatant of cultured decidual cells. These results suggest that IL-15 expression in human uterine mucosa corresponds to the fluctuation of uterine NK cells and that its production is hormonally controlled, especially by progesterone. |
| Databáze: | OpenAIRE |
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