Connexin57 is expressed in dendro-dendritic and axo-axonal gap junctions of mouse horizontal cells and its distribution is modulated by light
| Autor: | Luis Pérez de Sevilla Müller, Klaus Willecke, Konrad Schultz, Ulrike Janssen-Bienhold, Gerrit Hilgen, Stephan Sonntag, Karin Dedek, Petra Dirks, Reto Weiler, Jennifer Trümpler |
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| Rok vydání: | 2009 |
| Předmět: |
Light
Immunoelectron microscopy Blotting Western Outer plexiform layer In Vitro Techniques Retinal Horizontal Cells Biology Antibodies Connexins Mice chemistry.chemical_compound Axon terminal medicine Animals Electrical synapse Microscopy Immunoelectron Mice Knockout Retina Adaptation Ocular General Neuroscience Gap junction Gap Junctions Retinal Dendrites Immunohistochemistry Axons Mice Inbred C57BL medicine.anatomical_structure chemistry Ultrastructure Biophysics Neuroscience Photic Stimulation |
| Zdroj: | The Journal of Comparative Neurology. 513:363-374 |
| ISSN: | 1096-9861 0021-9967 |
| Popis: | Mouse horizontal cells are coupled by gap junctions composed of connexin57. These gap junctions are regulated by ambient light via multiple neuromodulators including dopamine. In order to analyze the distribution and structure of horizontal cell gap junctions in the mouse retina, and examine the effects of light adaptation on gap junction density, we developed antibodies that detect mouse retinal connexin57. Using immunohistochemistry in retinal slices, flatmounted retinas, and dissociated retinal cells, we showed that connexin57 is expressed in the dendrites and axon terminal processes of mouse horizontal cells. No staining was found in retinas of connexin57-deficient mice. Significantly more connexin57-positive puncta were found in the distal than in the proximal outer plexiform layer, indicating a higher level of expression in axon terminal processes than in the dendrites. We also examined the gap junctions using immunoelectron microscopy and showed that connexin57 does not form hemichannels in the horizontal cell dendritic tips. Light adaptation resulted in a significant increase in the number of connexin57-immunoreactive plaques in the outer plexiform layer, consistent with previously reported effects of light adaptation on connexin57 expression in the mouse retina. This study shows for the first time the detailed location of connexin57 expression within mouse horizontal cells, and provides the first ultrastructural data on mouse horizontal cell gap junctions. J. Comp. Neurol. 513:363–374 |
| Databáze: | OpenAIRE |
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