Visualization of microvascular proliferation as a tumor infiltration structure in rat glioma specimens using the diffraction-enhanced imaging in-plane CT technique
| Autor: | Gi-Hwan Choi, Tetsuya Yuasa, Eun-Ju Jeong, Jong-Ki Kim, Naoki Sunaguchi, Won-Seok Chang, Seung-Jun Seo, Ki-Hong Kim, Hong-Tae Kim, Masami Ando, Qingkai Huo |
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| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Necrosis Caudate nucleus Rats Sprague-Dawley Cell Line Tumor Glioma Image Processing Computer-Assisted medicine Animals Edema Radiology Nuclear Medicine and imaging Perivascular space Cell Proliferation Models Statistical Radiological and Ultrasound Technology Brain Neoplasms Chemistry Microcirculation Equipment Design Hyperplasia medicine.disease Rats medicine.anatomical_structure Acrylates Frontal lobe medicine.symptom Tomography X-Ray Computed Infiltration (medical) Neoplasm Transplantation Ex vivo |
| Zdroj: | Physics in Medicine and Biology. 57:1251-1262 |
| ISSN: | 1361-6560 0031-9155 |
| DOI: | 10.1088/0031-9155/57/5/1251 |
| Popis: | In order to study potent microenvironments of malignant gliomas with a high- resolution x-ray imaging technique, an injection orthotopic glioma model was made using the Sprague-Dawley rat. Total brain tissue, taken out as an ex vivo model, was examined with diffraction-enhanced imaging (DEI) computed tomography (CT) acquired with a 35 keV monochromatic x-ray. In the convolution-reconstructed 2D/3D images with a spatial resolution of 12.5 × 12.5 × 25 µm, distinction among necrosis, typical ring-shaped viable tumors, edemas and healthy tissues was clearly observed near the frontal lobe in front of the rat's caudate nucleus. Multiple microvascular proliferations (MVPs) were observed surrounding peritumoral edemas as a tumor infiltration structure. Typical dimensions of tubular MVPs were 130 (diameter) ×250 (length) µm with a partial sprout structure revealed in the 3D reconstructed image. Hyperplasia of cells around vessel walls was revealed with tumor cell infiltration along the perivascular space in microscopic observations of mild MVP during histological analysis. In conclusion, DEI-CT is capable of imaging potent tumor-infiltrating MVP structures surrounding high-grade gliomas. |
| Databáze: | OpenAIRE |
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