Loss of C. elegans BBS-7 and BBS-8 protein function results in cilia defects and compromised intraflagellar transport
| Autor: | William S. Davidson, Stephen J. Ansley, Mark J. Audeh, Jose L. Badano, Michael J. Reardon, Stephen R. Wicks, Ronald H.A. Plasterk, Allan K. Mah, Moe R. Mahjoub, Michel R. Leroux, Chunmei Li, Oliver E. Blacque, David L. Baillie, Jonathan McCarthy, Robert C. Johnsen, Lynne M. Quarmby, Philip L. Beales, Nicholas Katsanis |
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| Rok vydání: | 2004 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities BBSome Nerve Tissue Proteins Biology Animals Genetically Modified Bardet–Biedl syndrome Intraflagellar transport Genetics medicine Animals Basal body Cilia Caenorhabditis elegans Caenorhabditis elegans Proteins Adaptor Proteins Signal Transducing Chemotaxis Cilium Proteins Ciliary transition zone Helminth Proteins medicine.disease biology.organism_classification Research Papers Transport protein Cell biology Cytoskeletal Proteins Protein Transport Flagella Mutation sense organs Developmental Biology |
| Zdroj: | Genes & Development. 18:1630-1642 |
| ISSN: | 1549-5477 0890-9369 |
| DOI: | 10.1101/gad.1194004 |
| Popis: | Bardet-Biedl syndrome (BBS) is a genetically heterogeneous developmental disorder whose molecular basis is largely unknown. Here, we show that mutations in the Caenorhabditis elegans bbs-7 and bbs-8 genes cause structural and functional defects in cilia. C. elegans BBS proteins localize predominantly at the base of cilia, and like proteins involved in intraflagellar transport (IFT), a process necessary for cilia biogenesis and maintenance, move bidirectionally along the ciliary axoneme. Importantly, we demonstrate that BBS-7 and BBS-8 are required for the normal localization/motility of the IFT proteins OSM-5/Polaris and CHE-11, and to a notably lesser extent, CHE-2. We propose that BBS proteins play important, selective roles in the assembly and/or function of IFT particle components. Our findings also suggest that some of the cardinal and secondary symptoms of BBS, such as obesity, diabetes, cardiomyopathy, and learning defects may result from cilia dysfunction. |
| Databáze: | OpenAIRE |
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