Therapeutic effect of localized vibration on alveolar bone of osteoporotic rats

Autor: Cristina C. Teixeira, Edmund Khoo, Abdullah Almansour, Chinapa Sangsuwon, Mani Alikhani, Joo Y. Nho, José A. López, Serafim M. Oliveira, Jeanne M. Nervina, Mona Alikhani, Mohammad A. Hamidaddin, Sarah Alansari
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Anabolism
Bone density
genetic structures
Osteoporosis
Osteoclasts
Rats
Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
Fluorescence Microscopy
Bone Density
Animal Cells
Osteogenesis
Medicine and Health Sciences
Maxilla
Reproductive System Procedures
Connective Tissue Diseases
Musculoskeletal System
Connective Tissue Cells
Microscopy
Multidisciplinary
Lumbar Vertebrae
Physics
Classical Mechanics
Light Microscopy
Osteoblast
Femur Head
medicine.anatomical_structure
Connective Tissue
Physical Sciences
Ovariectomized rat
Medicine
Female
Anatomy
Cellular Types
Research Article
medicine.medical_specialty
Science
Ovariectomy
030209 endocrinology & metabolism
Surgical and Invasive Medical Procedures
Research and Analysis Methods
Vibration
03 medical and health sciences
Rheumatology
Osteoclast
Internal medicine
medicine
Animals
Bone
Dental alveolus
Skeleton
Nutrition
Surgical Excision
business.industry
Skull
Biology and Life Sciences
030206 dentistry
Cell Biology
X-Ray Microtomography
medicine.disease
Diet
Rats
Endocrinology
Biological Tissue
chemistry
Sclerostin
Alveolar Bone
business
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 1, p e0211004 (2019)
ISSN: 1932-6203
Popis: Objectives Vibration, in the form of high frequency acceleration (HFA), stimulates alveolar bone formation under physiologic conditions and during healing after dental extractions. It is not known if HFA has an anabolic effect on osteoporotic alveolar bone. Our objective is to determine if HFA has a regenerative effect on osteoporotic alveolar bone. Methods and materials Adult female Sprague-Dawley rats were divided into five groups: 1) Ovariectomized Group (OVX), 2) Sham-OVX Group that received surgery without ovariectomy, 3) OVX-HFA Group that was ovariectomized and treated daily with HFA, 4) OVX+Static Force Group that was ovariectomized and received the same force as HFA, but without vibration, and 5) Control Group that did not receive any treatment. All animals were fed a low mineral diet for 3 months. Osteoporosis was confirmed by micro-CT of the fifth lumbar vertebra and femoral head. HFA was applied to the maxillary first molar for 5 minutes/day for 28 and 56 days. Maxillae were collected for micro-CT, histology, fluorescent microscopy, protein and RNA analysis, and three-point bending mechanical testing. Results Micro-CT analysis revealed significant alveolar bone osteoporosis in the OVX group. Vibration restored the quality and quantity of alveolar bone to levels similar to the Sham-OVX group. Animals exposed to HFA demonstrated higher osteoblast activity and lower osteoclast activity. Osteogenic transcription factors (RUNX2, Foxo1, Osterix and Wnt signaling factors) were upregulated following vibration, while RANKL/RANK and Sclerostin were downregulated. HFA did not affect serum TRAcP-5b or CTx-1 levels. The osteogenic effect was highest at the point of HFA application and extended along the hemimaxillae this effect did not cross to the contra-lateral side. Conclusions Local application of vibration generated gradients of increased anabolic metabolism and decreased catabolic metabolism in alveolar bone of osteoporotic rats. Our findings suggest that HFA could be a predictable treatment for diminished alveolar bone levels in osteoporosis patients.
Databáze: OpenAIRE
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