Symbiotic N2 Fixation and Grain Yield of Endangered Kersting's Groundnut Landraces in Response to Soil and Plant Associated Bradyrhizobium Inoculation to Promote Ecological Resource-Use Efficiency
| Autor: | Sanjay K. Jaiswal, Mustapha Mohammed, Felix D. Dakora, Benjamin D. K. Ahiabor, E.N.K. Sowley |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Microbiology (medical) neglected and under-utilized legume lcsh:QR1-502 01 natural sciences Microbiology Bradyrhizobium Macrotyloma lcsh:Microbiology Rhizobia %Ndfa Symbiosis Genetic variability N-fixed Legume B value SSR markers biology Inoculation 04 agricultural and veterinary sciences biology.organism_classification Agronomy Soil water 040103 agronomy & agriculture 0401 agriculture forestry and fisheries δ15N 010606 plant biology & botany |
| Zdroj: | Frontiers in Microbiology, Vol 9 (2018) |
| DOI: | 10.3389/fmicb.2018.02105/full |
| Popis: | Kersting’s groundnut (Macrotyloma geocarpum Harms) is a neglected, endangered food and medicinal legume in Africa. Efforts to harness the benefits of the legume-Rhizobium symbiosis have focused on few major legumes to the neglect of underutilized ones such as Kersting’s groundnut. This study assessed plant growth, N-fixed and grain yield of five Kersting’s groundnut landraces in response to inoculation with Bradyrhizobium strain CB756 at two locations in the Northern Region of Ghana. The transferability of cowpea-derived Simple Sequence Repeat (SSR) markers to Kersting’s groundnut was also assessed. The symbiotic results revealed significant variation in nodulation, shoot biomass, δ15N, percent N derived from fixation, amount of N-fixed and soil N uptake. The cross-taxa SSR primers revealed monomorphic bands with sizes within the expected range in all the test Kersting’s groundnut landraces. The results of the aligned nucleotide sequences revealed marked genetic variability among the test landraces. Kersting’s groundnut was found to be a low N2-fixer, with 28 to 45% of its N derived from fixation at Nyankpala and 15 to 29% at Savelugu. Nitrogen contribution was 28-50 kg N-fixed.ha-1 at Nyankpala, and 12-32 kg N-fixed.ha-1 at Savelugu. Uninoculated plants of the Kersting’s groundnut landraces Puffeun, Dowie, Sigiri and Boli, respectively, contributed 22, 16, 13 and 15 kg N-fixed.ha-1 from symbiosis at Savelugu as opposed to 89, 82, 69 and 89 kg N.ha-1 from soil. Landrace Puffeun was highly compatible with the introduced strain CB756 based on δ15N and %Ndfa values, while Dowie, Funsi and Boli showed greater compatibility with native rhizobia in Ghanaian soils. The unimproved Kersting’s groundnut in association with soil microsymbionts could produce grain yield of 1137 to 1556 kg ha-1 at Nyankpala, and 921 to 1192 kg ha-1 at Savelugu. These findings suggest the need for further work to improve the efficiency of the Kersting’s groundnut-rhizobia symbiosis for increased grain yield and resource-use efficiency in cropping systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|