A conditional proteomics approach to identify proteins involved in zinc homeostasis
| Autor: | Takayuki Miki, Masaki Wakabayashi, Masashi Awa, Yuki Nishikawa, Itaru Hamachi, Shigeki Kiyonaka, Yasushi Ishihama |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Proteomics Proteome Recombinant Fusion Proteins Blotting Western Green Fluorescent Proteins chemistry.chemical_element Zinc 010402 general chemistry medicine.disease_cause Nitric Oxide 01 natural sciences Biochemistry 03 medical and health sciences Cell Line Tumor medicine Homeostasis Humans Molecular Biology High concentration Binding Sites Microscopy Confocal Staining and Labeling Zinc ion Cell Biology Glioma Physiological responses 0104 chemical sciences Cell biology Zinc homeostasis Oxidative Stress 030104 developmental biology chemistry Electrophoresis Polyacrylamide Gel Carrier Proteins Oxidative stress Biotechnology HeLa Cells Protein Binding |
| Zdroj: | Nature methods. 13(11) |
| ISSN: | 1548-7105 |
| Popis: | Zinc signaling and dynamics play significant roles in many physiological responses and diseases. To understand the physiological roles of zinc in detail, comprehensive identification of proteins under high concentration of mobile zinc ion is crucial. We developed a 'conditional proteomics' approach to identify proteins involved in zinc homeostasis based on a chemical proteomic strategy that utilizes designer zinc-responsive labeling reagents to tag such proteins and quantitative mass spectrometry for their identification. We used this method to elucidate zinc dyshomeostasis induced by nitric-oxide-triggered oxidative stress in glioma cells, and we unveiled dynamic changes of the zinc-related proteomes. Moreover, we characterized unknown zinc-rich vesicles generated by oxidative stress as endoplasmic-reticulum- and Golgi-related vesicles. |
| Databáze: | OpenAIRE |
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