Study of the Th1/Th2 balance, including IL-10 production,in cultures of peripheral blood mononuclear cells frombirch-pollen-allergic patients
| Autor: | R. Movérare, L. Elfman, G. Stålenheim, E. Björnsson |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Allergy Immunology Immunoglobulin E medicine.disease_cause Peripheral blood mononuclear cell Trees Interferon-gamma Th2 Cells Tetanus Toxoid Humans Immunology and Allergy Medicine Phytohemagglutinins Interleukin 5 Cells Cultured Interleukin 4 Interleukin-13 biology business.industry Rhinitis Allergic Seasonal Aeroallergen Allergens Th1 Cells medicine.disease Interleukin-10 Interleukin 10 Interleukin 13 Leukocytes Mononuclear biology.protein Tetradecanoylphorbol Acetate Female Interleukin-4 Interleukin-5 Mitogens Plant Lectins business |
| Zdroj: | Allergy. 55:171-175 |
| ISSN: | 1398-9995 0105-4538 |
| Popis: | Background: Excessive production of interleukin (IL)-4, IL-5, IL-10, and IL-13 is thought to be important in the development of allergy and asthma. The objective of this investigation was to study Th1/Th2-like cytokine profiles in vitro in seven patients allergic to birch pollen and six nonallergic controls during the birch-pollen season. Methods: Peripheral blood mononuclear cells (PBMC) were isolated and cultured with birch-pollen extract (BPE) or tetanus toxoid (TT) for 7 days, harvested, and restimulated with the mitogens phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (PMA) for 24 h. Cytokine production was determined by ELISA, and logarithmic cytokine ratios were compared between the two groups and between the antigens. Results: In the allergic group, the cultures prestimulated with BPE had a more Th2-like cytokine response than the TT-prestimulated cultures; i.e., lower IFN-γ and higher IL-10 production (P |
| Databáze: | OpenAIRE |
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