Cellular and molecular profiles of anterior nervous system regeneration in Diopatra claparedii Grube, 1878 (Annelida, Polychaeta)
| Autor: | Basri Saidi, Wan Iryani Wan Ismail, Othman Bin Haji Ross, Izwandy Idris, Mohd Hafizi Mahmud, Mohd Ulul Ilmie Ahmad Nazri, Mohd Noor Mat Isa, Zolkapli Ehsak |
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| Rok vydání: | 2021 |
| Předmět: |
Nervous system
0301 basic medicine Gene isoform Annelida Mitochondrion Transcriptome 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation medicine lcsh:Social sciences (General) lcsh:Science (General) Early regeneration Multidisciplinary biology Regeneration (biology) biology.organism_classification Polychaete Cell biology Diopatra 030104 developmental biology medicine.anatomical_structure Ultrastructure lcsh:H1-99 030217 neurology & neurosurgery lcsh:Q1-390 |
| Zdroj: | Heliyon, Vol 7, Iss 2, Pp e06307-(2021) |
| ISSN: | 2405-8440 |
| Popis: | The polychaete Diopatra claparedii Grube, 1878 is among those organisms successfully carrying out full body regeneration, including the whole nervous system. Thus, D. claparedii potentially can be regarded for the nervous system regeneration (NSR) study. However, data on the property of its nervous system and the NSR profile are still lacking. In this study, we investigated the morphology of D. claparedii anterior nervous system (ANS) and examined the cellular and molecular profiles on its early anterior NSR. The nervous system of D. claparedii consists of a symmetry brain with nerves branching off, circumpharyngeal connectives that connect the brain and nerve cord as well as obvious segmental ganglia. Moreover, we identified changes in the cellular condition of the ganglionic cells in the regenerating tissue, such as the accumulation of lysosomes and lipofuscins, elongated mitochondria and multiple nucleoli. Furthermore, mRNA of tissues at two regenerating stages, as well as intact tissue (non-regenerating), were sequenced with Illumina sequencer. We identified from these tissues 37,248 sequences, 18 differential expressed proteins of which upregulated were involved in NSR with noelin-like isoform X2 turned up to be the highest being expressed. Our results highlight the cellular and molecular changes during early phase of NSR, thus providing essential insights on regeneration within Annelida and understanding the neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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