Balancing precision versus cohort transcriptomic analysis of acute and recovery phase of viral bronchiolitis
| Autor: | Sophia Y. Lunt, Dominic Sanfilippo, André S. Bachmann, Ruchir Gupta, Mara L. Leimanis, Jeremy W. Prokop, Sarah S. Comstock, Eric J. Kort, Rosemary Olivero, Nicholas L. Hartog, Katie L. Uhl, Marie Adams, Surender Rajasekaran, Caleb Bupp |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Physiology Respiratory Syncytial Virus Infections Severity of Illness Index Minor Histocompatibility Antigens Transcriptome 03 medical and health sciences 0302 clinical medicine Immune system Physiology (medical) medicine Bronchiolitis Viral Humans 030212 general & internal medicine business.industry Cell Biology medicine.disease Precision medicine 030104 developmental biology medicine.anatomical_structure Virus Diseases Bronchiolitis Respiratory Syncytial Virus Human Immunology Cohort business Research Article Recovery phase Respiratory tract |
| Zdroj: | Am J Physiol Lung Cell Mol Physiol |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.00440.2020 |
| Popis: | Viral infections affecting the lower respiratory tract place enormous burdens on hospitals. As neither vaccines nor antiviral agents exist for many viruses, understanding risk factors and outcomes in each patient using minimally invasive analysis, such as blood, can lead to improved health care delivery. A cohort of PAXgene RNA sequencing of infants admitted with moderate or severe acute bronchiolitis and respiratory syncytial virus were compared with case-control statistical analysis and cohort-based outlier mapping for precision transcriptomics. Patients with severe bronchiolitis had signatures connected to the immune system, interferon signaling, and cytokine signaling, with marked sex differences in XIST, RPS4Y1, KDM5D, and LINC00278 for severity. Several patients had unique secondary infections, cytokine activation, immune responses, biological pathways, and immune cell activation, highlighting the need for defining patient-level transcriptomic signatures. Balancing relative contributions of cohort-based biomarker discoveries with patient’s biological responses is needed to understand the totality of mechanisms of adverse outcomes in viral bronchiolitis. |
| Databáze: | OpenAIRE |
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