Confrontation of the 'Dual Tracer' Indirect Method With Direct Ileal Sampling for Indispensable Amino Acid Digestibility of Sunflower Isolate in Humans
| Autor: | Robert Benamouzig, Nadezda Khodorova, Claire Gaudichon, Romain Tessier, Romain Kapel, Alain Quinsac, Juliane Calvez, Gheorghe Airinei, Daniel Tomé, Olivier Galet |
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| Přispěvatelé: | Physiologie de la Nutrition et du Comportement Alimentaire (PNCA (UMR 0914)), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Terres Inovia, Laboratoire Réactions et Génie des Procédés (LRGP), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Groupe Avril - Sofiprotéol |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Nutrition and Dietetics Chromatography 030309 nutrition & dietetics Direct assessment Enrichment ratio Less invasive Medicine (miscellaneous) Sampling (statistics) 030209 endocrinology & metabolism Sunflower Test protein Amino acid 03 medical and health sciences 0302 clinical medicine chemistry Methods Dual tracer [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Food Science |
| Zdroj: | Current Developments in Nutrition Nutrition 2021 Nutrition 2021, Jun 2021, online, United States. ⟨10.1093/cdn/nzab048_019⟩ Curr Dev Nutr |
| Popis: | OBJECTIVES: The direct assessment of ileal samples of amino acid (AA) digestibility is invasive in humans. A less invasive but indirect method, namely « dual tracer » was recently developed. It relies on the plasma isotopic enrichment ratio of two labeled protein, a reference protein or alternately AAs labeled with (13)C and the test protein labeled with (15)N. This recent method has not yet been challenged against direct measurement of ileal digestibility. METHODS: Seven healthy volunteers were intubated with naso-ileal tube. Every 30min for 4h, they ingested sunflower biscuits containing a total of 25g of (15)N intrinsically labeled sunflower protein isolate. They also ingested 60g chocolate containing a total of 400mg of a mix of (13)C algal individual AAs. Ileal contents were collected continuously for 8h following the first meal and plasma was sampled every 30min for 4h and hourly between 4 and 8h. (15)N and (13)C indispensable amino acid (IAA) ileal digestibility were determined by measuring (15)N and (13)C enrichment in AAs by GC-C-IRMS and AA content by UHPLC in ileal effluent. Plasma and meal (15)N and (13)C IAA enrichment were measured by GC-C-IRMS. Isotopic (15)N/(13)C ratio were determined using area under the curve value for each isotope. RESULTS: Using direct ileal sampling, average IAA ileal digestibility was: (i) 88.5 ± 5.0% for sunflower isolate ((15)N) with values ranged from 85.8 ± 5.1% for threonine to 91.1 ± 5.8% for methionine, and (ii) 97.6 ± 1.7% for free AAs (13C) with values ranged from 95.9 ± 2.3% for lysine to 98.8 ± 0.8% for phenylalanine. With the “dual tracer” method, digestibility of isoleucine, leucine, threonine and valine was significantly lower than with ileal determination (from 7.9% for threonine to 24.3% for leucine), Methionine and phenylalanine values were aberrant (over 100%) For lysine, the difference between the two methods was not statistically different (4.7%, p = 0.49). CONCLUSIONS: With our methodological conditions, the “dual tracer” method provides physiological values for most IAA except methionine and phenylalanine. However, values were low compared to ileal digestibility (about 10%) and interindividual variability was high. This less invasive method is promising but requires methodological improvements. FUNDING SOURCES: French Research National Agency (ANR), financial support of SOFIPROTEOL under the FASO Project PRODIAL. |
| Databáze: | OpenAIRE |
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