Pathogenic features of heterotrophic plate count bacteria from drinking-water boreholes
| Autor: | Carlos Bezuidenhout, Rialet Pieters, Suranie Horn |
|---|---|
| Přispěvatelé: | 10075399 - Pieters, Rialet, 12540110 - Bezuidenhout, Cornelius Carlos, 21080097 - Horn, Suranie Rachel |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Cytotoxicity 030106 microbiology Bacillus cereus Bacillus subtilis Bacterial Physiological Phenomena Microbiology 03 medical and health sciences Bacterial Proteins RNA Ribosomal 16S Humans Waste Management and Disposal Water Science and Technology Human duodenal cells Bacteria Virulence biology Bacillus pumilus Aeromonas taiwanensis Drinking Water Pathogenecity Pseudomonas fungi Public Health Environmental and Occupational Health Simulated gastric fluid Sequence Analysis DNA Extracellular enzymes biology.organism_classification Bacterial Load Anti-Bacterial Agents Aeromonas hydrophila 030104 developmental biology Infectious Diseases HPC bacteria Lecithinase |
| Popis: | Evidence suggests that heterotrophic plate count (HPC) bacteria may be hazardous to humans with weakened health. We investigated the pathogenic potential of HPC bacteria from untreated borehole water, consumed by humans, for: their haemolytic properties, the production of extracellular enzymes such as DNase, proteinase, lipase, lecithinase, hyaluronidase and chondroitinase, the effect simulated gastric fluid has on their survival, as well as the bacteria's antibiotic-susceptible profile. HuTu-80 cells acted as model for the human intestine and were exposed to the HPC isolates to determine their effects on the viability of the cells. Several HPC isolates were α- or β-haemolytic, produced two or more extracellular enzymes, survived the SGF treatment, and showed resistance against selected antibiotics. The isolates were also harmful to the human intestinal cells to varying degrees. A novel pathogen score was calculated for each isolate. Bacillus cereus had the highest pathogen index: the pathogenicity of the other bacteria declined as follows: Aeromonas taiwanensis>Aeromonas hydrophila>Bacillus thuringiensis>Alcaligenes faecalis > Pseudomonas sp. >Bacillus pumilus>Brevibacillus sp. >Bacillus subtilis>Bacillus sp. These results demonstrated that the prevailing standards for HPCs in drinking water may expose humans with compromised immune systems to undue risk. |
| Databáze: | OpenAIRE |
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