Gel electrophoresis of human sperm: a simple method for evaluating sperm protein quality
| Autor: | Buee Luc, Sigala Julien, Mitchell Valérie, Fernandez-Gomez Francisco-Jose, Jumeau Fanny, Béhal Hélène, Sergeant Nicolas, Eddarkaoui Sabiha, Duban-Deweer Sophie |
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| Přispěvatelé: | Gamétogenèse et Qualité du Gamète - ULR 4308 (GQG), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université de Lille, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system Electrophorèse unidimensionnelle Urology education Motility Sperm protein Biology Flagellum World health Andrology 03 medical and health sciences 0302 clinical medicine Fibrous sheath Sperme Sperm motility health care economics and organizations reproductive and urinary physiology Gel electrophoresis lcsh:R5-920 030219 obstetrics & reproductive medicine Gaine fibreuse urogenital system Correction [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Sperm 030104 developmental biology Reproductive Medicine One-dimensional PAGE lcsh:Medicine (General) Research Article |
| Zdroj: | Basic and Clinical Andrology Basic and clinical andrology Basic and clinical andrology, BioMed Central, 2018, 28 (1), pp.10. ⟨10.1186/s12610-018-0076-0⟩ Basic and Clinical Andrology, Vol 28, Iss 1, Pp 1-8 (2018) Basic and clinical andrology, 2018, 28 (1), pp.10. ⟨10.1186/s12610-018-0076-0⟩ |
| ISSN: | 2051-4190 |
| DOI: | 10.1186/s12610-018-0076-0⟩ |
| Popis: | Background The limitations of conventional sperm analyses have highlighted the need for additional means of evaluating sperm quality. Methods In a study of a cohort of 245 men with known conventional sperm parameters, one-dimensional PAGE was used to monitor protein content and quality in samples from individual ejaculates. Results The sperm protein content varied markedly from sample to another, especially in the high-molecular-weight range. The intensity of the 80–110 kDa bands was correlated with progressive motility (r = 0.15, p = 0.015) and was significantly higher (p = 0.0367) in the group of men with conventional parameters above the World Health Organization’s 2010 reference values than in the group with at least one subnormal parameter (i.e. semen volume, sperm concentration, sperm count per ejaculate, progressive motility, proportion of normal forms or multiple anomaly index below the lower reference value). Using mass spectrometry, the 80–110 kDa bands were found to correspond primarily to three proteins from the flagellum’s fibrous sheath: A-kinase anchor protein 4, A-kinase anchor protein 3, and spermatogenic cell-specific type 1 hexokinase. Conclusion One-dimensional PAGE constitutes a simple, rapid, reliable, inexpensive method for analyzing proteins associated with sperm motility in individual human ejaculates. |
| Databáze: | OpenAIRE |
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