Proteomics and Visual Health Research: Proteome of the Human Sclera Using High-Resolution Mass Spectrometry
| Autor: | Krishna R Murthy, Yashwanth Subbannayya, Kunal Kumar Singh, Sunita Dalal, Cynthia Arunachalam, Sneha M. Pinto, Varshasnata Mohanty, Mohammed Altaf Najar, Shyamjith Manikkoth, Hilda Karuppiah, Thottethodi Subrahmanya Keshava Prasad, Bhuvaragavan Sreeramulu, Sandeep Kasaragod |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
0301 basic medicine Proteome genetic structures Context (language use) Computational biology Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Genetics medicine Humans Molecular Biology Gene ontology Computational Biology eye diseases Sclera Family member 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Molecular Medicine Eye disorder Synaptopodin sense organs Biotechnology |
| Zdroj: | OMICS: A Journal of Integrative Biology. 23:98-110 |
| ISSN: | 1557-8100 |
| DOI: | 10.1089/omi.2018.0185 |
| Popis: | Eye disorders and resulting visual loss are major public health problems affecting millions of people worldwide. In this context, the sclera is an opaque, thick outer coat covering more than 80% of the eye, and essential in maintaining the shape of the eye and protecting the intraocular contents against infection and the external environment. Despite efforts undertaken to decipher the scleral proteome, the functional and structural picture of the sclera still remain elusive. Recently, proteomics has arisen as a powerful tool that enables identification of proteins playing a critical role in health and disease. Therefore, we carried out an in-depth proteomic analysis of the human scleral tissue using a high-resolution Orbitrap Fusion Tribrid mass spectrometer. We identified 4493 proteins using SequestHT and Mascot as search algorithms in Proteome Discoverer 2.1. Importantly, the proteins, including radixin, synaptopodin, paladin, netrin 1, and kelch-like family member 41, were identified for the first time in human sclera. Gene ontology analysis unveiled that the majority of proteins were localized to the cytoplasm and involved in cell communication and metabolism. In sum, this study offers the largest catalog of proteins identified in sclera with the aim of facilitating their contribution to diagnostics and therapeutics innovation in visual health and autoimmune disorders. This study also provides a valuable baseline for future investigations so as to map the dynamic changes that occur in sclera in various pathological conditions. |
| Databáze: | OpenAIRE |
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