Transplantation of choroid plexus epithelial cells into contusion-injured spinal cord of rats

Autor: Masanori Fukushima, Norihiko Nakano, Atsushi Yokota, Masayoshi Ohta, Toru Noda, Yoshihisa Suzuki, Chizuka Ide, Yoshihiro Yamada, Kenji Kanekiyo
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Pathology
Rats
Sprague-Dawley
0302 clinical medicine
GAP-43 Protein
Medicine
Gap-43 protein
Spinal cord injury
Cells
Cultured
Glial fibrillary acidic protein
biology
axonal regeneration
medicine.anatomical_structure
Neurology
Immunohistochemistry
Choroid plexus
Female
medicine.symptom
Locomotion
Research Article
medicine.medical_specialty
trophic factor
Contusions
Green Fluorescent Proteins
Transfection
Lesion
03 medical and health sciences
Developmental Neuroscience
Glial Fibrillary Acidic Protein
Animals
Spinal Cord Injuries
business.industry
Epithelial Cells
Spinal cord
medicine.disease
choroid plexus epithelial cell
Axons
Rats
Transplantation
Disease Models
Animal
Microscopy
Electron
030104 developmental biology
Animals
Newborn
Choroid Plexus
biology.protein
Neurology (clinical)
business
030217 neurology & neurosurgery
transplantation
Zdroj: Restorative Neurology and Neuroscience
ISSN: 1878-3627
0922-6028
Popis: Purpose: The effect of the transplantation of choroid plexus epithelial cells (CPECs) on locomotor improvement and tissue repair including axonal extension in spinal cord lesions was examined in rats with spinal cord injury (SCI). Methods: CPECs were cultured from the choroid plexus of green fluorescent protein (GFP)-transgenic rats, and transplanted directly into the contusion-injured spinal cord lesions of rats of the same strain. Locomotor behaviors were evaluated based on BBB scores every week after transplantation until 4 weeks after transplantation. Histological and immunohistochemical examinations were performed at 2 days, and every week until 5 weeks after transplantation. Results: Locomotor behaviors evaluated by the BBB score were significantly improved in cell-transplanted rats. Numerous axons grew, with occasional interactions with CPECs, through the astrocyte-devoid areas. These axons exhibited structural characteristics of peripheral nerves. GAP-43-positive axons were found at the border of the lesion 2 days after transplantation. Cavity formation was more reduced in cell-transplanted than control spinal cords. CPECs were found within the spinal cord lesion, and sometimes in association with astrocytes at the border of the lesion until 2 weeks after transplantation. Conclusion: The transplantation of CPECs enhanced locomotor improvement and tissue recovery, including axonal regeneration, in rats with SCI.
Databáze: OpenAIRE
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