Enumeration and Characterization of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization
| Autor: | María L. Espejo-Cruz, Sandra González-Rubio, Juan J. Espejo, Javier M. Zamora-Olaya, Rafael M. Alejandre-Altamirano, María Prieto-Torre, Clara I. Linares, Marta Guerrero-Misas, Pilar Barrera-Baena, Antonio Poyato-González, Marina Sánchez-Frías, María D. Ayllón, Manuel L. Rodríguez-Perálvarez, Manuel de la Mata, Gustavo Ferrín |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
liquid biopsy
Liquid biopsy Hepatocellular carcinoma Organic Chemistry Circulating tumor cells circulating cancer stem cells spheroids General Medicine hepatocellular carcinoma transarterial chemoembolization circulating tumor cells Circulating cancer stem cells Transarterial chemoembolization Catalysis Computer Science Applications Inorganic Chemistry Physical and Theoretical Chemistry Spheroids Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Science, 24(3), 2558 (2023) International Journal of Molecular Sciences Volume 24 Issue 3 Pages: 2558 |
| Popis: | Circulating tumor cells (CTCs), and particularly circulating cancer stem cells (cCSC), are prognostic biomarkers for different malignancies and may be detected using liquid biopsies. The ex vivo culture of cCSCs would provide valuable information regarding biological aggressiveness and would allow monitoring the adaptive changes acquired by the tumor in real time. In this prospective pilot study, we analyzed the presence of EpCAM+ CTCs using the IsoFlux system in the peripheral blood of 37 patients with hepatocellular carcinoma undergoing transarterial chemoembolization (TACE). The average patient age was 63.5 ± 7.9 years and 91.9% of the patients were men. All patients had detectable CTCs at baseline and 20 patients (54.1%) showed CTC aggregates or clusters in their peripheral blood. The increased total tumor diameter (OR: 2.5 (95% CI: 1.3–4.8), p = 0.006) and the absence of clusters of CTCs at baseline (OR: 0.2 (95% CI: 0.0–1.0), p = 0.049) were independent predictors of a diminished response to TACE. Culture of cCSC was successful in five out of thirty-three patients, mostly using negative enrichment of CD45− cells, ultra-low adherence, high glucose, and a short period of hypoxia followed by normoxia. In conclusion, the identification of clusters of CTCs before TACE and the implementation of standardized approaches for cCSC culture could aid to predict outcomes and to define the optimal adjuvant therapeutic strategy for a true personalized medicine in hepatocellular carcinoma. |
| Databáze: | OpenAIRE |
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