PCR Analysis of cry7 Genes in Bacillus thuringiensis by the Five Conserved Blocks of Toxins

Autor:	Robert Manasherob, Yoel Margalith, Ze'ev Barak, Eitan Ben-Dov, Arieh Zaritsky
Rok vydání:	2001
Předmět:	Bacterial Toxins Molecular Sequence Data Bacillus thuringiensis Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology DNA sequencing law.invention Conserved sequence Hemolysin Proteins Bacterial Proteins law Amino Acid Sequence Peptide sequence Gene Conserved Sequence Polymerase chain reaction DNA Primers Genetics chemistry.chemical_classification Bacillus thuringiensis Toxins biology General Medicine Amplicon biology.organism_classification Amino acid Endotoxins chemistry Genes Bacterial
Zdroj:	Current Microbiology. 42:96-99
ISSN:	1432-0991 0343-8651
DOI:	10.1007/s0028403336
Popis:	An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65f8c09375d109b81367de86c11b5339 https://doi.org/10.1007/s0028403336 Zobrazit plný text záznamu Full text from SpringerLink