A spontaneous induction of fetal membrane prostaglandin production precedes clinical labour
| Autor: | S. A. Alvi, Murdoch G. Elder, N. L. Brown, Phillip R. Bennett, M.H.F. Sullivan |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Time Factors Endocrinology Diabetes and Metabolism medicine.medical_treatment Extraembryonic Membranes Biology Dinoprostone Endocrinology Pregnancy In vivo Fetal membrane Culture Techniques Internal medicine medicine Humans Cyclooxygenase Inhibitors Prostaglandin E2 Analysis of Variance Fetus Aspirin Stimulation Chemical In vitro Endotoxins Cytokine Membrane Eicosanoid Prostaglandin-Endoperoxide Synthases Labor Onset Female lipids (amino acids peptides and proteins) Interleukin-1 medicine.drug |
| Zdroj: | Journal of Endocrinology. 157:R1-R6 |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1677/joe.0.157r001 |
| Popis: | Fetal membranes from term human pregnancies produce prostaglandins, and may respond to bacterial endotoxin or interleukin-1 beta (IL-1 beta) with increased prostaglandin E2 (PGE2) production. The effects of endotoxin persisted for up to 24 h, whereas those of IL-1 beta were maximal 4-8 h after addition. The maximum levels of PGE2 (200-350 pg/ml) were similar in all experiments, and were independent of the stimulus used. Not all tissues responded to these stimuli; those which did not had basal levels of PGE2 production of 200-350 pg/ml, which was not further increased by endotoxin or IL-1 beta. The basal production from these tissues was therefore similar to the maximal production from those tissues which responded to endotoxin or IL-1 beta. The high basal production of PGE2 was attributed to prior in vivo activation of the membranes such that PGE2 synthesis could not be further stimulated in vitro. Overnight pretreatment with aspirin decreased basal PGE2 production from these activated membranes to < 100 pg/ml/4 h during subsequent culture in aspirin-free medium. Both endotoxin and IL-1 beta increased PGE2 production from the activated aspirin-pretreated membranes during this culture time, but this was transient as after 12 h of culture basal PGE2 production rose to over 200 pg/ml despite aspirin pretreatment. |
| Databáze: | OpenAIRE |
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