Stimulation of (3H)glucosamine incorporation into the cell surface of Amoeba by pinocytosis

Autor:	Richard J. Winzler, J.F. Danielli, Howard J. Allen
Rok vydání:	1976
Předmět:	Glucosamine food.ingredient Elution Pinocytosis Cell Cell Membrane Stimulation Cell Biology Biology Cell Fractionation Hydrolysate Amoeba (genus) chemistry.chemical_compound food medicine.anatomical_structure Biochemistry chemistry Galactosamine medicine Animals Amoeba
Zdroj:	Experimental cell research. 100(2)
ISSN:	0014-4827
Popis:	Suspensions of amoebae were treated with [3H]glucosamine either before or after exposure to solutions of KCl or Alcian blue. Several hours later, the cell surface was isolated. Comparison of specific activities of control and experimental material showed that pinocytosis caused a specific increase in the amount of [3H]glucosamine incorporated into the cell surface fraction. Ion-exchange chromatography of acid hydrolysates of pooled radiolabeled surface fractions showed that all of the label eluted at positions corresponding to glucosamine and galactosamine.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65f22f5681dc9806ebbaf5074c3bacfe https://pubmed.ncbi.nlm.nih.gov/939264 Zobrazit plný text záznamu