Three-dimensional point spread function estimation method for mid-wave infrared microscope imaging

Autor: Anselmo Jara, Sergio N. Torres, Guillermo Machuca, Pablo Coelho, Laura A. Viafora
Rok vydání: 2022
Předmět:
Zdroj: Applied Optics. 61:8467
ISSN: 2155-3165
1559-128X
DOI: 10.1364/ao.470508
Popis: A three-dimensional point spread function experimental estimation method based on the system’s focal plane array spatial local impulse response of a mid-wave infrared microscope is presented. The method uses several out-of-focus two-dimensional point spread function planes to achieve a single three-dimensional point spread function of the whole microscope’s optical spreading, expanding the limits of infrared optical technology by one dimension. This technique includes stages of image acquisition, nonuniformity correction, filtering, and multi-planar reconstruction steps, and its effectiveness is demonstrated on biological sample image restoration by means of a multi-planar refocusing application.
Databáze: OpenAIRE