5‐Hydroxymethylcytosine profiling from genomic and cell‐free DNA for colorectal cancers patients
| Autor: | Yi Sui, Ping-Hong Zhou, Jing Lin, Jiaxiuyu Liu, Ming-Yan Cai, Xingyu Lu, Yuehong Cui, Yan Zhu, Yun-Shi Zhong, Yanqun Song, Shengli Lin, Ping-Ting Gao |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Epigenomics Adenoma precancerous adenoma Colorectal cancer Biology 5‐hydroxymethylcytosine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine hmC‐Seal Humans Epigenetics Liquid biopsy Gene 5-Hydroxymethylcytosine Regulation of gene expression Cell Biology Original Articles DNA Neoplasm Genomics Sequence Analysis DNA DNA Methylation medicine.disease Gene Expression Regulation Neoplastic 030104 developmental biology Gene Ontology Cell-free fetal DNA chemistry colon cancer 030220 oncology & carcinogenesis Cancer research 5-Methylcytosine Molecular Medicine Original Article Colorectal Neoplasms Cell-Free Nucleic Acids Precancerous Conditions |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | 5‐Hydroxymethylcytosine (5hmC) is a DNA modification that is generated by the oxidation of 5‐methylcytosine (5mC) in a reaction catalyzed by the ten‐eleven translocation (TET) family enzymes. It tends to mark gene activation and affects a spectrum of developmental and disease‐related biological processes. In this manuscript, we present a 5hmC selective chemical labelling technology (hmC‐Seal) to capture and sequence 5hmC‐containing DNA fragments with low input. We tested 10 tumour/adjacent colon cancer tissues and 10 tumour/healthy plasma samples. Furthermore, we tested if this methodology could generate the 5hmC differential genes among cancer patients, healthy controls and precancerous adenoma patients from plasma. Robust cancer‐specific epigenetic signatures were identified for colon cancers. The results show that 5hmC is mainly distributed in gene active regions. The results also indicate the potential application of 5hmC change signals in early stage of colon cancer, even show potential in the diagnosis of precancerous adenoma. We demonstrated the robustness of the 5hmC‐Seal method in tissue and cell‐free DNA (cfDNA) as potential biomarkers. Moreover, this study provides the potential value and feasibility of 5hmC‐Seal approach on colorectal cancer (CRC) early detection. We believe this strategy could be an effective liquid biopsy‐based diagnosis and a potential prognosis method for colon cancer using cfDNA. |
| Databáze: | OpenAIRE |
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