Overexpression of FOXM1 is associated with EMT and is a predictor of poor prognosis in non-small cell lung cancer
| Autor: | Xiao-Di Gong, Wen-Ying Zhang, Yue-Hui Guo, Mei Zhao, Hai-Hua Yuan, Zeng-Qiang Qu, Fei-Fei Kong, Jiong-Yi Wang, Bin Jiang, Jing Shi, You-Long Zhu, Feng Liu |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Cancer Research Epithelial-Mesenchymal Transition Biology Metastasis Carcinoma Non-Small-Cell Lung Carcinoma medicine Humans Lung cancer Aged Neoplasm Staging Tissue microarray Oncogene Forkhead Box Protein M1 Cancer Ribosomal Protein S6 Kinases 70-kDa Forkhead Transcription Factors General Medicine Middle Aged medicine.disease Prognosis Molecular medicine Gene Expression Regulation Neoplastic Oncogene Protein v-akt Oncology Lymphatic Metastasis Cancer research FOXM1 Female Signal Transduction |
| Zdroj: | Oncology reports. 31(6) |
| ISSN: | 1791-2431 |
| Popis: | Forkhead box M1 (FOXM1), a member of the Fox family of transcriptional factors, is considered to be an independent predictor of poor survival in many solid cancers. However, the underlying mechanism is not yet clear. The aim of the present study was to investigate the clinical significance of the correlation between FOXM1 and epithelial-mesenchymal transition (EMT) in non-small cell lung carcinoma and the possible mechanism responsible for FOXM1-induced EMT and metastasis. In the present study, expression levels of FOXM1 and EMT indicator proteins were determined by tissue microarray (TMA) and immunohistochemical staining, western blotting and reverse transcription-PCR (RT-PCR). Other cellular and molecular approaches including gene transfection, small interfering RNA (siRNA), and migration and invasion assays were utilized. Our results demonstrated that FOXM1 overexpression was statistically significantly associated with a higher TNM stage (p=0.036), lymph node metastasis (p=0.009) and a positive smoking history of the patients (p=0.044). Additionally, high expression of FOXM1 correlated with loss of E-cadherin expression (p |
| Databáze: | OpenAIRE |
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