Extracellular Acidification Related to the Stimulation of Catecholamines Release by Strontium in the Bovine Adrenal Medulla
| Autor: | J E B Pinto, P N Viglione, K Pereyra, C F Reyes-Toso |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
inorganic chemicals
medicine.medical_specialty Physiology Stimulation In Vitro Techniques Catecholamines Physiology (medical) Internal medicine Extracellular medicine Animals Magnesium Secretion Gallopamil Medulla Osmole Osmotic concentration Chemistry Osmolar Concentration General Medicine Hydrogen-Ion Concentration Calcium Channel Blockers Endocrinology medicine.anatomical_structure Adrenal Medulla Strontium Potassium Catecholamine Cattle Extracellular Space Adrenal medulla medicine.drug |
| Zdroj: | Archives of Physiology and Biochemistry. 104:833-837 |
| ISSN: | 1744-4160 1381-3455 |
| DOI: | 10.1076/apab.104.7.833.13112 |
| Popis: | The possible modifications of extracellular pH associated with the secretion of catecholamines evoked by the introduction of 2.2 mM Sr2+ to a Ca(2+)-free, buffer-free, Locke solution were investigated in decorticated perfused bovine adrenal glands. A progressive and reversible decrease of external pH accompanied the catecholamine release promoted by Sr(2+)-introduction into the perfusion fluid. This extracellular acid shift was practically undetected when the chromaffin tissue was stimulated by the addition of Sr2+ to a buffered medium. Both the secretory response as well as the extracellular pH drop mediated by Sr(2+)-introduction to a Ca(2+)-free, buffer-free, Locke solution were markedly inhibited by methoxyverapamil (0.3 mM), Mg2+ (20 mM) and hyperosmolarity (750 mOsm). The exposure of the adrenal medulla to a Ca(2+)-free, buffer-free, high-K+ solution containing 2.2 mM Sr2+ for 6 min promoted a significant enhancement of both the secretory response and the acidification of the perfusates compared with the responses evoked by Sr2+ in a 5.6 mM K+ medium. These results are consistent with the existence of a close relationship between extracellular acidification and the release of catecholamines triggered by the introduction of Sr2+ to the perfusion fluid. |
| Databáze: | OpenAIRE |
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